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PMID: 2841167 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of the porcine nuclear factor I (NFI) gene.

FEBS letters ·Vol. 236 ·No. 1 ·1988-08-15 ·Pages 27-32

Meisterernst M, Rogge L, Donath C, Gander I, Lottspeich F, Mertz R, Dobner T, Föckler R, Stelzer G, Winnacker EL

Abstract

This study describes the isolation of a major portion of the gene for nuclear factor I (NFI) including its 5'-flanking region with transcriptional start sites. We screened a porcine liver, genomic DNA library in phage EMBL3A with synthetic oligonucleotides derived from tryptic and cyanogen-bromide peptide sequences obtained from purified NFI protein. The NFI gene is present as a single copy in porcine DNA.

MeSH Terms
Amino Acid Sequence Animals Autoradiography Base Sequence CCAAT-Enhancer-Binding Proteins Cloning, Molecular DNA/genetics DNA Restriction Enzymes DNA-Binding Proteins/genetics,isolation & purification Endonucleases Exons Genes Introns Liver/analysis Molecular Sequence Data NFI Transcription Factors Nuclear Proteins Nucleic Acid Hybridization Single-Strand Specific DNA and RNA Endonucleases Transcription Factors Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human DNA Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Meisterernst M
Institut für Biochemie der Universität München, FRG.
Rogge L
Donath C
Gander I
Lottspeich F
Mertz R
Dobner T
Föckler R
Stelzer G
Winnacker E L
Article Info
Journal
FEBS letters
Abbr.
FEBS Lett
ISSN
0014-5793
Published
1988-08-15
Pages
27-32
Language
English
Region
England
NLM ID
0155157
Subset
IM
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