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PMID: 2836375 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evidence for endonucleolytic cleavages in decay of lacZ and lacI mRNAs.

Journal of bacteriology ·Vol. 170 ·No. 6 ·1988-06-00 ·Pages 2860-5

Subbarao MN, Kennell D

Abstract

S1 nuclease mapping revealed lacZ mRNA molecules whose 5' and 3' ends were internal to the transcription start and consistent with cleavages at pyrimidine-adenosine bonds 20 to 50 nucleotides apart. With the net 5'-to-3' direction known, lacZ mRNA is probably degraded by sequential cleavages of naked mRNA at vulnerable sites exposed by transit of the last translating ribosome.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Electrophoresis, Polyacrylamide Gel Endonucleases/metabolism Molecular Sequence Data Nucleic Acid Conformation RNA, Messenger/metabolism Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
Bacterial Proteins RNA, Messenger Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Subbarao M N
Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110.
Kennell D
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.6.2860-2865.1988
Published
1988-06-00
Pages
2860-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211215
Subset
IM
Grants
NIGMS NIH HHS · GM34127 · United States
External Links
PubMed source DOI: 10.1128/jb.170.6.2860-2865.1988 PMC Full Text View journal details
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