Home LiteratureArticle Details
PMID: 2835398 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L. An abundant transcript induced by transformation of fibroblasts.

The Journal of clinical investigation ·Vol. 81 ·No. 5 ·1988-05-00 ·Pages 1621-9

Joseph LJ, Chang LC, Stamenkovich D, Sukhatme VP

Abstract

Transfection of an activated rat oncogene into NIH3T3 fibroblasts leads to transformation and induction of a metastatic phenotype. To identify genes whose activation might mediate these processes, we used a differential screening strategy. A 1.5-kb transcript is induced fiftyfold, constitutes 1% of ras transformed cell messenger RNA (mRNA) and is the most abundantly induced message in these cells. Our sequence data shows that it encodes murine cathepsin L, a potent collagenolytic and elastinolytic lysosomal enzyme. The murine clone was used to isolate human cathepsin L complementary DNA (cDNA) clones. The complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L are presented and compared to other papain family cysteine proteinases. Northern analysis shows that both human and murine cathepsin L probes hybridize to a 1.5-kb transcript in several tissues, but also to a 4-kb transcript in human kidney. These clones will facilitate studies of the structure, expression, and function of cathepsin L, including its unexpected upregulation in transformation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cathepsins/genetics Cell Line, Transformed Cloning, Molecular DNA/genetics DNA Restriction Enzymes Deoxyribonuclease EcoRI Enzyme Precursors/genetics Fibroblasts Genes, ras Humans Mice Molecular Sequence Data Nucleic Acid Hybridization RNA, Messenger/genetics Transcription, Genetic Transfection Transformation, Genetic
Chemicals
Enzyme Precursors RNA, Messenger DNA DNA Restriction Enzymes Deoxyribonuclease EcoRI Cathepsins preprocathepsin L
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Joseph L J
Howard Hughes Medical Institute, Department of Medicine, University of Chicago, Illinois 60637.
Chang L C
Stamenkovich D
Sukhatme V P
References (60)
60 references, click to expand
  1. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  2. Transformation of mammalian cells with genes from procaryotes and eucaryotes.
    Cell. 1979 Apr;16(4):777-85 PMID: 222468
  3. Tumor promoters and Kirsten sarcoma virus increase synthesis of a secreted glycoprotein by regulating levels of translatable mRNA.
    Cell. 1980 Feb;19(2):449-55 PMID: 6244111
  4. Cathepsin B, Cathepsin H, and cathepsin L.
    Methods Enzymol. 1981;80 Pt C:535-61 PMID: 7043200
  5. Action of rat liver cathepsin L on collagen and other substrates.
    Biochem J. 1982 Feb 1;201(2):367-72 PMID: 7082295
  6. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes.
    Nature. 1982 Jul 22;298(5872):343-7 PMID: 6283384
  7. Exocytosis regulates urinary acidification in turtle bladder by rapid insertion of H+ pumps into the luminal membrane.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4327-31 PMID: 6289300
  8. Identification and characterization of cells deficient in the mannose 6-phosphate receptor: evidence for an alternate pathway for lysosomal enzyme targeting.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):775-9 PMID: 6298775
  9. Homology of amino acid sequences of rat liver cathepsins B and H with that of papain.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3666-70 PMID: 6574504
  10. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides.
    J Mol Biol. 1983 Jun 25;167(2):391-409 PMID: 6345794
  11. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  12. Flat revertants isolated from Kirsten sarcoma virus-transformed cells are resistant to the action of specific oncogenes.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5602-6 PMID: 6604274
  13. Activation of mouse genes in transformed cells.
    Cell. 1983 Sep;34(2):557-67 PMID: 6311432
  14. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  15. Activation of a Qa/Tla class I major histocompatibility antigen gene is a general feature of oncogenesis in the mouse.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):756-60 PMID: 6318117
  16. How signal sequences maintain cleavage specificity.
    J Mol Biol. 1984 Feb 25;173(2):243-51 PMID: 6423828
  17. Uteroferrin has N-asparagine-linked high-mannose-type oligosaccharides that contain mannose 6-phosphate.
    Proc Natl Acad Sci U S A. 1984 May;81(10):2985-9 PMID: 6587337
  18. Monoclonal antibodies of predefined specificity detect activated ras gene expression in human mammary and colon carcinomas.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5227-31 PMID: 6382261
  19. Isolation of the gene encoding the human T-lymphocyte differentiation antigen Leu-2 (T8) by gene transfer and cDNA subtraction.
    Proc Natl Acad Sci U S A. 1984 Dec;81(24):7688-92 PMID: 6440142
  20. Processing and lysosomal localization of a glycoprotein whose secretion is transformation stimulated.
    J Cell Biol. 1985 Feb;100(2):535-44 PMID: 3968177
  21. Thiol proteases. Comparative studies based on the high-resolution structures of papain and actinidin, and on amino acid sequence information for cathepsins B and H, and stem bromelain.
    J Mol Biol. 1985 Mar 20;182(2):317-29 PMID: 3889350
  22. Tumorigenic and metastatic properties of "normal" and ras-transfected NIH/3T3 cells.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3698-701 PMID: 3858844
  23. Epidermal growth factor and oncogenes induce transcription of the same cellular mRNA in rat fibroblasts.
    EMBO J. 1985 Jun;4(6):1435-40 PMID: 3875482
  24. Rat mammary gland in culture secretes a stable high molecular weight form of cathepsin L.
    Biochem Biophys Res Commun. 1985 Aug 30;131(1):402-7 PMID: 4038303
  25. Harvey ras induction of metastatic potential depends upon oncogene activation and the type of recipient cell.
    Am J Pathol. 1985 Oct;121(1):1-8 PMID: 3901774
  26. Expression of ras oncogene p21 in prostate cancer.
    N Engl J Med. 1986 Jan 16;314(3):133-7 PMID: 2417118
  27. The tumor promoter phorbol 12-myristate 13-acetate induces a program of altered gene expression similar to that induced by platelet-derived growth factor and transforming oncogenes.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):357-60 PMID: 3455773
  28. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  29. The major excreted protein of transformed fibroblasts is an activable acid-protease.
    J Biol Chem. 1986 Feb 5;261(4):1760-5 PMID: 3511049
  30. Trafficking of lysosomal enzymes in normal and disease states.
    J Clin Invest. 1986 Jan;77(1):1-6 PMID: 3003148
  31. Cellular ras oncogene expression and cell cycle measured by flow cytometry in hematopoietic cell lines.
    Blood. 1986 Mar;67(3):676-81 PMID: 3511984
  32. Primary rat embryo cells transformed by one or two oncogenes show different metastatic potentials.
    Science. 1986 Apr 11;232(4747):223-7 PMID: 3456644
  33. Degradation of myocardial structural proteins in myocardial infarcted dogs is reduced by Ep459, a cysteine proteinase inhibitor.
    Biol Chem Hoppe Seyler. 1986 Jan;367(1):39-45 PMID: 3006724
  34. Intracellular protein catabolism: state of the art.
    FEBS Lett. 1986 Mar 31;198(2):181-93 PMID: 3007210
  35. Elastinolytic activity of human cathepsin L.
    Biochem J. 1986 Feb 1;233(3):925-7 PMID: 3518704
  36. Activities of cathepsins B and L in isolated nephron segments from proteinuric and nonproteinuric rats.
    Am J Physiol. 1986 Jun;250(6 Pt 2):F1055-62 PMID: 3717347
  37. Suppression of tumorigenicity with continued expression of the c-Ha-ras oncogene in EJ bladder carcinoma-human fibroblast hybrid cells.
    Proc Natl Acad Sci U S A. 1986 Jul;83(14):5209-13 PMID: 3523486
  38. Experimental metastasis in nude mice of NIH 3T3 cells containing various ras genes.
    Proc Natl Acad Sci U S A. 1986 Jul;83(14):5277-81 PMID: 3523488
  39. Close relationship of the major excreted protein of transformed murine fibroblasts to thiol-dependent cathepsins.
    Cancer Res. 1986 Sep;46(9):4590-3 PMID: 3755373
  40. Regulation of the transcript for a lysosomal protein: evidence for a gene program modified by platelet-derived growth factor.
    Mol Cell Biol. 1985 Oct;5(10):2582-9 PMID: 3915532
  41. Nucleotide and predicted amino acid sequences of cloned human and mouse preprocathepsin B cDNAs.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7721-5 PMID: 3463996
  42. Cloning and characterization of a mouse cysteine proteinase.
    J Biol Chem. 1986 Nov 5;261(31):14697-703 PMID: 3533924
  43. Cathepsin L inactivates alpha 1-proteinase inhibitor by cleavage in the reactive site region.
    J Biol Chem. 1986 Nov 5;261(31):14748-51 PMID: 3490478
  44. 17 beta-estradiol regulates and v-Ha-ras transfection constitutively enhances MCF7 breast cancer cell interactions with basement membrane.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8182-6 PMID: 2946041
  45. Phosphorylation, glycosylation, and proteolytic activity of the 52-kD estrogen-induced protein secreted by MCF7 cells.
    J Cell Biol. 1987 Feb;104(2):253-62 PMID: 3543022
  46. Activation of growth factor secretion in tumorigenic states of breast cancer induced by 17 beta-estradiol or v-Ha-ras oncogene.
    Proc Natl Acad Sci U S A. 1987 Feb;84(3):837-41 PMID: 2880347
  47. The N-terminal amino acid sequences of the heavy and light chains of human cathepsin L. Relationship to a cDNA clone for a major cysteine proteinase from a mouse macrophage cell line.
    Biochem J. 1986 Dec 1;240(2):373-7 PMID: 3545185
  48. Expression of H-ras correlates with metastatic potential: evidence for direct regulation of the metastatic phenotype in 10T1/2 and NIH 3T3 cells.
    Mol Cell Biol. 1987 Feb;7(2):830-7 PMID: 3102946
  49. The major ras induced protein in NIH3T3 cells is cathepsin L.
    Nucleic Acids Res. 1987 Apr 10;15(7):3186 PMID: 3550705
  50. Transformation by Rous sarcoma virus induces a novel gene with homology to a mitogenic platelet protein.
    Cell. 1987 May 8;49(3):321-8 PMID: 3032449
  51. Beta 1-6 branching of Asn-linked oligosaccharides is directly associated with metastasis.
    Science. 1987 May 1;236(4801):582-5 PMID: 2953071
  52. Lysosomal enzyme precursors in coated vesicles derived from the exocytic and endocytic pathways.
    J Cell Biol. 1987 Jun;104(6):1743-8 PMID: 2884223
  53. Activation of the intramyofibral autophagic-lysosomal system in muscular dystrophy.
    Am J Pathol. 1987 Jun;127(3):461-6 PMID: 3296770
  54. G proteins: control of exocytosis.
    Nature. 1987 Jul 9-15;328(6126):112-3 PMID: 3110624
  55. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  56. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  57. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  58. Transformation-dependent secretion of a low molecular weight protein by murine fibroblasts.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2767-71 PMID: 351614
  59. Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridization.
    Cell. 1979 Feb;16(2):443-52 PMID: 378392
  60. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI113497
Published
1988-05-00
Pages
1621-9
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC442598
Subset
IM
Databases
GENBANK
M20495, M20496
External Links
PubMed source DOI: 10.1172/JCI113497 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2