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PMID: 2829212 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and expression of rat 1,25-dihydroxyvitamin D3 receptor cDNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 4 ·1988-02-00 ·Pages 1005-9

Burmester JK, Maeda N, DeLuca HF

Abstract

The cDNA for the 1,25-dihydroxyvitamin D3 receptor has been isolated by screening a rat kidney lambda gt11 expression library with a mixture of three previously identified anti-receptor monoclonal antibodies. Protein produced from this cDNA reacts with each of the three monoclonal antibodies but does not react with an antibody specific for the porcine receptor or an irrelevant antibody. In addition, the expressed protein specifically binds 1,25-dihydroxyvitamin D3 but does not bind estradiol, progesterone, testosterone, cortisol, or aldosterone. The nucleotide sequence for this cDNA has been determined and used to deduce the amino acid sequence of the protein. This amino acid sequence contains a region that matches perfectly the partial amino acid sequence determined for the receptor isolated from porcine intestine. This amino acid sequence also closely matches the reported amino acid sequence of the putative DNA-binding finger of the avian 1,25-dihydroxyvitamin D3 receptor.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal/immunology Base Sequence Calcitriol/metabolism DNA/genetics DNA, Recombinant Molecular Sequence Data Rats Receptors, Calcitriol Receptors, Steroid/genetics,immunology,metabolism Recombinant Fusion Proteins/metabolism Steroids/metabolism
Chemicals
Antibodies, Monoclonal DNA, Recombinant Receptors, Calcitriol Receptors, Steroid Recombinant Fusion Proteins Steroids DNA Calcitriol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Burmester J K
Department of Biochemistry, University of Wisconsin-Madison 53706.
Maeda N
DeLuca H F
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13 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.4.1005
Published
1988-02-00
Pages
1005-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279689
Subset
IM
Grants
NIDDK NIH HHS · DK-14881 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.4.1005 PMC Full Text View journal details
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