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PMID: 2829171 Published · ppublish English Journal Article

Hepatitis B virus suppresses expression of human beta-interferon.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 1 ·1988-01-00 ·Pages 252-6

Twu JS, Lee CH, Lin PM, Schloemer RH

Abstract

To determine whether hepatitis B virus (HBV) regulates the expression of the human beta-interferon gene, a series of recombinant bovine papilloma virus plasmids containing the human interferon gene and various fragments of the HBV genome were constructed and used to transform C127 cells, a murine fibroblast line. Analysis of the DNA from transformed C127 cells indicated that the interferon gene was intact and that the plasmids replicated as stable multicopy elements. The 1828-base-pair BamHI HBV DNA fragment containing the core antigen gene, but not the 2755-base-pair Bgl II HBV DNA fragment encoding both the surface antigen and the X antigen, suppressed the production of human beta-interferon. No effect by any of the recombinant plasmids on the synthesis of murine interferon was detected. The suppression of human beta-interferon by HBV occurs via a trans-acting factor. A frameshift mutation within the HBV core gene alleviates the inhibitory activity; thus we infer that the core protein is this factor or is crucially associated with this activity.

MeSH Terms
Animals Bovine papillomavirus 1/genetics Cell Transformation, Viral Cells, Cultured Cloning, Molecular DNA Restriction Enzymes Genes Genes, Viral Hepatitis B virus/genetics Humans Interferon-gamma/genetics Mice Mutation Papillomaviridae/genetics Plasmids Transcription, Genetic Transfection
Chemicals
Interferon-gamma DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Twu J S
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46223.
Lee C H
Lin P M
Schloemer R H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.1.252
Published
1988-01-00
Pages
252-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279522
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.1.252 PMC Full Text View journal details
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