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PMID: 2827209 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A protoplast transformation system for Listeria sp.

Plasmid ·Vol. 18 ·No. 1 ·1987-07-00 ·Pages 89-92

Vicente MF, Baquero F, Perez-Diaz JC

Abstract

We describe protoplast transformation of members of the genus Listeria with plasmid DNA. Shuttle vectors able to replicate in both Escherichia coli and Listeria have been constructed by fusing the E. coli plasmid pUC8 with the Bacillus subtilis plasmids pBD9 and pBD10.

MeSH Terms
DNA Replication DNA Restriction Enzymes Escherichia coli/genetics Genetic Vectors Listeria/genetics,physiology Phenotype Plasmids Protoplasts/physiology Transformation, Bacterial
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vicente M F
Servicio de Microbiologia, Hospital Ramon y Cajal, Madrid, Spain.
Baquero F
Perez-Diaz J C
Article Info
Journal
Plasmid
Abbr.
Plasmid
ISSN
0147-619X
DOI
10.1016/0147-619x(87)90082-5
Published
1987-07-00
Pages
89-92
Language
English
Region
United States
NLM ID
7802221
Subset
IM
External Links
PubMed source DOI: 10.1016/0147-619x(87)90082-5 View journal details
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