Home LiteratureArticle Details
PMID: 282613 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of a tumor cell surface antigen from spontaneously transformed BALB/c mouse fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 12 ·1978-12-00 ·Pages 5912-6

Kamm AR, Grimes WJ

Abstract

This work describes the detection, isolation, and partial characterization of a BALB/c mouse fibroblast cell surface antigen. This antigen migrates as a polypeptide of approximately 100,000 daltons in a discontinuous sodium dodecyl sulfate/polyacrylamide gel electrophoresis system, can be labeled by either lactoperoxidase-catalyzed cell surface 125I iodination or metabolic incorporation of [3H]glucosamine, and can be isolated by concanavalin A affinity chromatography. This cell surface glycoprotein is antigenic in BALB/c mice and has been correlated with the rejection of immunogenic tumor cells. Also, antiserum specific for Moloney leukemia virus precipitates the 100,000-dalton cell surface protein from viral and immunogenic spontaneous transformants. This virus-related antigen comigrates on sodium dodecyl sulfate gels with the major iodinated cell surface protein of these transformants. Rabbit antiserum to the purified antigen demonstrates a marked preference for the surfaces of immunogenic tumor cells as compared with normal cells and nonimmunogenic tumor cells.

MeSH Terms
Animals Antigens, Neoplasm/isolation & purification Antigens, Surface/isolation & purification Antigens, Viral/analysis Cell Line Cell Transformation, Neoplastic/analysis Cross Reactions Glycoproteins/immunology,isolation & purification Mice Mice, Inbred BALB C Molecular Weight Moloney murine leukemia virus/immunology Neoplasms, Experimental/immunology
Chemicals
Antigens, Neoplasm Antigens, Surface Antigens, Viral Glycoproteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kamm A R
Grimes W J
References (23)
23 references, click to expand
  1. A comparison of membrane components of normal and transformed BALB/c cells.
    Biochemistry. 1977 Jun 28;16(13):2902-8 PMID: 195596
  2. Demonstration of different glycosylated antigens in C-type virus-transformed and infected rat cells by antiserum to murine leukemia virus.
    Cancer Res. 1977 Apr;37(4):1165-9 PMID: 191179
  3. Chemical and immunological studies of cell surfaces from normal and transformed cells.
    J Supramol Struct. 1977;6(3):449-64 PMID: 338994
  4. Purification and structural characterisation of human HLA-linked B-cell antigens.
    Nature. 1977 Jul 21;268(5617):213-8 PMID: 301990
  5. Carbohydrate compositions of normal, spontaneously transformed, and virally transformed cells derived from BALB/c mice.
    Cancer Res. 1976 Nov;36(11 Pt 1):3905-10 PMID: 975038
  6. Concanavalin A-induced agglutination and tumorigenicity in virally and spontaneously transformed cells derived from BALB-c mice.
    Cancer Res. 1974 Jun;34(6):1408-12 PMID: 4363657
  7. A rapid and simple method for the detection of mycoplasma and other intracellular contaminants.
    Experientia. 1975 SEP 15;31(9):1111-2 PMID: 1175759
  8. Biochemical characterization of tumor-specific cell surface antigens on avian oncornavirus transformed cells.
    Virology. 1975 Aug;66(2):481-91 PMID: 168686
  9. The role of cell surface changes in RNA tumor virus-transformed cells.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 2:1181-5 PMID: 169020
  10. Immunologic, virologic, and genetic aspects of mammary tumor virus-induced cell-surface antigens: presence of these antigens and the Thy 1.2 antigen on murine mammary gland and tumor cells.
    J Natl Cancer Inst. 1975 Jun;54(6):1323-33 PMID: 166186
  11. The cellular events associated with regression and progression of murine (Moloney) sarcomas.
    Int J Cancer. 1974 Jan 15;13(1):54-63 PMID: 4856384
  12. Mycoplasma contamination of animal cell cultures: a simple, rapid detection method.
    Exp Cell Res. 1972 Sep;74(1):99-109 PMID: 4672478
  13. Vasoformative sarcomas arising from BALB/3T3 cells attached to solid substrates.
    Cancer Res. 1976 May;36(5):1626-33 PMID: 1268823
  14. Surface polypeptides of the Chinese hamster ovary cell; an immunochemical study.
    Can J Biochem. 1976 Feb;54(2):185-91 PMID: 944075
  15. Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.
    J Biol Chem. 1971 Oct 25;246(20):6328-34 PMID: 5127429
  16. Human lymphocyte membrane (HL-A) alloantigens: isolation, purification and properties.
    J Immunol. 1969 Aug;103(2):282-92 PMID: 5803787
  17. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain.
    Exp Cell Res. 1977 Feb;104(2):255-62 PMID: 65285
  18. Moloney leukemia virus-induced cell surface antigen: detection and characterization in sodium dodecyl sulfate gels.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5270-4 PMID: 74831
  19. Detergent solubilization, purification, and separation of specificities of HLA antigens from a cultured human lymphoblastoid line, RPMI 4265.
    J Biol Chem. 1977 Jul 10;252(13):4682-93 PMID: 68955
  20. An abnormal membrane glycoprotein associated with malignancy in a wide range of different tumours.
    Proc R Soc Lond B Biol Sci. 1978 Apr 13;201(1142):87-106 PMID: 27794
  21. Antibody response of mice to chemically induced tumors.
    Proc Natl Acad Sci U S A. 1978 Feb;75(2):955-8 PMID: 204940
  22. Induction of two transformation-sensitive membrane polypeptides in normal fibroblasts by a block in glycoprotein synthesis or glucose deprivation.
    Cell. 1977 Aug;11(4):941-7 PMID: 196769
  23. Isolation and immunological characterization of a glucose-regulated fibroblast cell surface glycoprotein and its nonglycosylated precursor.
    Cell. 1978 Jan;13(1):139-40 PMID: 340047
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.12.5912
Published
1978-12-00
Pages
5912-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393086
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.12.5912 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2