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PMID: 28230814 Published · epublish English Journal Article Review Research Support, Non-U.S. Gov't

m1A Post-Transcriptional Modification in tRNAs.

Biomolecules ·Vol. 7 ·No. 1 ·2017-00-21

Oerum S, Dégut C, Barraud P, Tisné C

Abstract

To date, about 90 post-transcriptional modifications have been reported in tRNA expanding their chemical and functional diversity. Methylation is the most frequent post-transcriptional tRNA modification that can occur on almost all nitrogen sites of the nucleobases, on the C5 atom of pyrimidines, on the C2 and C8 atoms of adenosine and, additionally, on the oxygen of the ribose 2'-OH. The methylation on the N1 atom of adenosine to form 1-methyladenosine (m1A) has been identified at nucleotide position 9, 14, 22, 57, and 58 in different tRNAs. In some cases, these modifications have been shown to increase tRNA structural stability and induce correct tRNA folding. This review provides an overview of the currently known m1A modifications, the different m1A modification sites, the biological role of each modification, and the enzyme responsible for each methylation in different species. The review further describes, in detail, two enzyme families responsible for formation of m1A at nucleotide position 9 and 58 in tRNA with a focus on the tRNA binding, m1A mechanism, protein domain organisation and overall structures.

Keywords
1-methyladenosine Trm10 Trm6–Trm61 TrmI Trmt10C m1A tRNA methylation
MeSH Terms
Adenosine/analogs & derivatives,metabolism Animals Enzymes/metabolism Humans Methylation Models, Molecular RNA Processing, Post-Transcriptional RNA, Transfer/chemistry,genetics,metabolism
Chemicals
Enzymes 1-methyladenosine RNA, Transfer Adenosine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Oerum Stephanie
Institut de Biologie Physico-chimique (IBPC), CNRS, UMR 8261 CNRS/Université Paris Diderot, 13 rue Pierre et Marie Curie, Paris 75005, France. oerum@ibpc.fr.
Dégut Clément
Institut de Biologie Physico-chimique (IBPC), CNRS, UMR 8261 CNRS/Université Paris Diderot, 13 rue Pierre et Marie Curie, Paris 75005, France. c.degut@gmail.com.
Barraud Pierre
Institut de Biologie Physico-chimique (IBPC), CNRS, UMR 8261 CNRS/Université Paris Diderot, 13 rue Pierre et Marie Curie, Paris 75005, France. pierre.barraud@cnrs.fr.
Tisné Carine
Institut de Biologie Physico-chimique (IBPC), CNRS, UMR 8261 CNRS/Université Paris Diderot, 13 rue Pierre et Marie Curie, Paris 75005, France. carine.tisne@cnrs.fr.
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Article Info
Journal
Biomolecules
Abbr.
Biomolecules
ISSN
2218-273X
DOI
10.3390/biom7010020
Email
oerum@ibpc.fr
c.degut@gmail.com
pierre.barraud@cnrs.fr
carine.tisne@cnrs.fr
Published
2017-00-21
Epub
2017-00-21
Language
English
Region
Switzerland
NLM ID
101596414
PMCID
PMC5372732
Subset
IM
External Links
PubMed source DOI: 10.3390/biom7010020 PMC Full Text View journal details
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