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PMID: 2822942 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A highly conserved sequence in H1 histone genes as an oligonucleotide hybridization probe: isolation and sequence of a duck H1 gene.

Journal of molecular evolution ·Vol. 25 ·No. 4 ·1987-00-00 ·Pages 361-70

Tönjes R, Doenecke D

Abstract

A 3.5-kb HindIII fragment of a histone gene cluster was isolated from a recombinant phage out of a duck genomic library. This DNA contains a duck H1 gene and its flanking sequences. The hybridization probe, which was used to screen for the H1 gene, had been designed on the basis of a comparative analysis of available H1 gene and protein data. Most H1 histones contain repeated motifs in their C-terminal domain, and these form part of an octapeptide (ser pro lys lys ala lys lys pro) that is highly conserved in many H1 histone proteins. A comparison of the duck H1 described here with two different published chicken H1 histone sequences reveals conservative amino acid exchanges at 22 (of 217 and 218, respectively) positions. The homology is maintained at the flanking sequences, and includes the putative H1 histone gene-specific signal structures and the established 3' stem and loop structures and the CAAGA box. The duck H1 gene and its flanking sequence have been found in identical arrangements in two recombinant bacteriophages, but minor sequence variations and genomic Southern blotting after HindIII digestion suggest that we have either isolated alleles of this genome segment or that the gene described may occur twice per haploid duck genome.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution DNA/genetics,isolation & purification DNA Restriction Enzymes Ducks/genetics Genes Histones/genetics Molecular Sequence Data Nucleic Acid Hybridization Oligodeoxyribonucleotides/chemical synthesis Species Specificity
Chemicals
Histones Oligodeoxyribonucleotides DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tönjes R
Physiologische Chemie I, Philipps-Universität, Marburg/Lahn, FRG.
Doenecke D
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF02603121
Published
1987-00-00
Pages
361-70
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
Databases
GENBANK
X06128
External Links
PubMed source DOI: 10.1007/BF02603121 View journal details
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