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PMID: 2820709 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

cDNA sequences of Torpedo marmorata acetylcholinesterase: primary structure of the precursor of a catalytic subunit; existence of multiple 5'-untranslated regions.

The EMBO journal ·Vol. 6 ·No. 7 ·1987-07-00 ·Pages 1865-73

Sikorav JL, Krejci E, Massoulié J

Abstract

cDNA clones coding for a catalytic subunit of acetylcholinesterase were isolated from cDNA libraries constructed from Torpedo marmorata electric organ. The nucleotide sequence of the cloned cDNAs codes for a 599-amino acid precursor containing a 24-amino acid signal peptide. This primary structure has been compared with the sequences of Torpedo californica and Drosophila melanogasta acetylcholinesterases, and with that of human butyrylcholinesterase. Genomic blot experiments carried out with cDNA restriction fragments used as hybridization probes are in agreement with the existence of a single gene coding for the different catalytic subunits of Torpedo acetylcholinesterase. Unexpectedly, we observed multiple 5'-untranslated regions, which may contain several initiation codons.

MeSH Terms

Acetylcholinesterase/genetics Amino Acid Sequence Animals Base Sequence Butyrylcholinesterase/genetics Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Drosophila melanogaster/enzymology Electric Organ/enzymology Humans Macromolecular Substances Molecular Sequence Data Protein Biosynthesis Sequence Homology, Nucleic Acid Species Specificity Torpedo

Chemicals

Macromolecular Substances DNA Acetylcholinesterase Butyrylcholinesterase DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Sikorav J L

Laboratoire de Neurobiologie, Ecole Normale Supérieure, Paris, France.

Krejci E

Massoulié J

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27 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1987-07-00

Pages

1865-73

Language

English

Region

England

NLM ID

8208664

PMCID

PMC553570

Subset

Databases

GENBANK

X05497

External Links

PubMed source PMC Full Text View journal details

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cDNA sequences of Torpedo marmorata acetylcholinesterase: primary structure of the precursor of a catalytic subunit; existence of multiple 5'-untranslated regions.

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