An enzymatic assay for picomole levels of phosphatidate.

Analytical biochemistry ·Vol. 180 ·No. 1 ·1989-07-00 ·Pages 24-7

Bocckino SB, Wilson P, Exton JH

Abstract

An assay for phosphatidate that is sensitive, specific, and highly reproducible is described. Phosphatidate is transacylated with methylamine and the glycerol 3-phosphate produced is measured enzymatically. The assay is linear from 50 to 1500 pmol of phosphatidate and can be used to quantitate phosphatidate in small amounts of biological materials.

MeSH Terms

Animals Chromatography, High Pressure Liquid Chromatography, Thin Layer Glycerol-3-Phosphate Dehydrogenase (NAD+) Glycerolphosphate Dehydrogenase In Vitro Techniques Liver/analysis,cytology Male Methylamines Microchemistry NAD Phosphatidic Acids/analysis Phosphorus Isotopes Rats

Chemicals

Methylamines Phosphatidic Acids Phosphorus Isotopes NAD methylamine Glycerolphosphate Dehydrogenase Glycerol-3-Phosphate Dehydrogenase (NAD+)

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bocckino S B

Howard Hughes Medical Institute, Nashville, Tennessee.

Wilson P

Exton J H

Article Info

Journal

Analytical biochemistry

Abbr.

Anal Biochem

ISSN

0003-2697

DOI

10.1016/0003-2697(89)90083-3

Published

1989-07-00

Pages

24-7

Language

English

Region

United States

NLM ID

0370535

Subset

External Links

PubMed source DOI: 10.1016/0003-2697(89)90083-3 View journal details

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An enzymatic assay for picomole levels of phosphatidate.

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