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PMID: 2813355 Published · ppublish English Journal Article

Cloning of the cDNA encoding the myosin heavy chain of a vertebrate cellular myosin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 20 ·1989-10-00 ·Pages 7726-30

Shohet RV, Conti MA, Kawamoto S, Preston YA, Brill DA, Adelstein RS

Abstract

The complete amino acid sequence of a vertebrate cellular myosin heavy chain (MHC; 1,959 amino acids, 226 kDa) has been deduced by using cDNA clones from a chicken intestinal epithelial cell library. RNA blot analysis of kidney, spleen, brain, liver, and intestinal epithelial cells as well as smooth muscle cells from the aorta and gizzard indicates the presence of a 7.3-kilobase (kb) message that is larger than the message for chicken smooth and striated muscle MHC. The chicken intestinal epithelial cell MHC shows overall similarity in primary structure to other MHCs in the areas of the reactive thiol residues and in areas contributing to the ATP binding site and actin binding site. The globular head domain is followed by an alpha-helical coiled-coil region, and as in smooth muscle MHC there is a short uncoiled sequence at the carboxyl terminus of the molecule. Comparison of amino acid sequences in the rod regions between human and chicken cellular MHCs shows a remarkable 92% identity.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Chickens Cloning, Molecular DNA/genetics Epithelium/metabolism Gene Library Humans Molecular Sequence Data Myosin Subfragments/genetics Nucleic Acid Hybridization Organ Specificity Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Myosin Subfragments DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Shohet R V
Laboratory of Molecular Cardiology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
Conti M A
Kawamoto S
Preston Y A
Brill D A
Adelstein R S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.20.7726
Published
1989-10-00
Pages
7726-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298143
Subset
IM
Databases
GENBANK
M26510
External Links
PubMed source DOI: 10.1073/pnas.86.20.7726 PMC Full Text View journal details
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