Home LiteratureArticle Details
PMID: 279918 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Temperature dependence of calcium-induced fusion of sonicated phosphatidylserine vesicles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 9 ·1978-09-00 ·Pages 4325-8

Sun ST, Day EP, Ho JT

Abstract

We have measured the temperature dependence calcium-induced fusion of sonicated phosphatidylserine vesicles. The vesicles were incubated in the presence of calcium at a specified temperature until the resulting aggregation or fusion process had gone to completion. EDTA was then added and the resulting final size of the vesicle population was measured by using dynamic light scattering. This final size was plotted against incubation temperature to show the temperature dependence of calcium-induced fusion. This curve has a peak near 11 degrees C which may be associated with the phase transition of the sonicated phosphatidylserine vesicles in the presence of calcium prior to the aggregation or fusion process.

MeSH Terms
Animals Calcium Cattle Cell Fusion Kinetics Lasers Liposomes Membranes/physiology Models, Biological Molecular Weight Phosphatidylserines Scattering, Radiation Sonication Temperature
Chemicals
Liposomes Phosphatidylserines Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sun S T
Day E P
Ho J T
References (21)
21 references, click to expand
  1. The interaction of spectrin - actin and synthetic phospholipids.
    Biochim Biophys Acta. 1977 Oct 17;470(2):152-60 PMID: 911826
  2. Specificity of Ca2+ and Mg2+ binding to phosphatidylserine vesicles and resultant phase changes of bilayer membrane structure.
    Biochim Biophys Acta. 1978 Jan 19;506(2):281-7 PMID: 620034
  3. Phase transitions in phospholipid model membranes of different curvature.
    Biochim Biophys Acta. 1978 Jan 19;506(2):183-91 PMID: 620027
  4. Studies on membrane fusion. I. Interactions of pure phospholipid membranes and the effect of myristic acid, lysolecithin, proteins and dimethylsulfoxide.
    Biochim Biophys Acta. 1976 Oct 5;448(2):254-64 PMID: 971433
  5. Crystalline anhydrous Ca-phosphatidylserine bilayers.
    Biochem Biophys Res Commun. 1976 May 23;76(2):267-74 PMID: 1027430
  6. Dynamic light scattering study of calcium-induced fusion in phospholipid vesicles.
    Biochim Biophys Acta. 1977 Nov 1;470(3):503-8 PMID: 411510
  7. A calorimetric examination of stable and fusing lipid bilayer vesicles.
    Biochim Biophys Acta. 1977 May 2;466(3):402-10 PMID: 857888
  8. The influence of pH, Ca2+ and protein on the thermotropic behaviour of the negatively charged phospholipid, phosphatidylglycerol.
    Biochim Biophys Acta. 1974 Mar 29;339(3):432-7 PMID: 4834678
  9. Effect of sonication on the structure of lecithin bilayers.
    Biochemistry. 1972 Nov 21;11(24):4573-81 PMID: 4654145
  10. Fusion of dimyristoyllecithin vesicles as studied by proton magnetic resonance spectroscopy.
    Biochemistry. 1974 Mar 12;13(6):1122-6 PMID: 4814717
  11. Membrane fusion.
    Biochim Biophys Acta. 1973 Dec 28;300(4):421-65 PMID: 4360422
  12. A monolayer and freeze-etching study of charged phospholipids. I. Effects of ions and pH on the ionic properties of phosphatidylglycerol and lysylphosphatidylglycerol.
    Chem Phys Lipids. 1974 May;12(3):201-19 PMID: 4135141
  13. Phospholipid model membranes. I. Structural characteristics of hydrated liquid crystals.
    Biochim Biophys Acta. 1967 Sep 9;135(4):624-38 PMID: 4167394
  14. Fusion of fatty acid containing lecithin vesicles.
    Biochemistry. 1975 Apr 22;14(8):1790-5 PMID: 1125198
  15. Calorimetric and freeze-etch study of the influence of Mg2+ on the thermotropic behaviour of phosphatidylglycerol.
    Chem Phys Lipids. 1975 Feb;14(1):97-101 PMID: 1122567
  16. Electrostatic effects on lipid phase transitions: membrane structure and ionic environment.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):214-9 PMID: 4521052
  17. Phase transitions and phase separations in phospholipid membranes induced by changes in temperature, pH, and concentration of bivalent cations.
    Biochemistry. 1975 Jan 14;14(1):152-61 PMID: 234017
  18. Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles.
    Biochim Biophys Acta. 1975 Jul 3;394(3):483-91 PMID: 805602
  19. Fusion of dipalmitoylphosphatidylcholine vesicle membranes induced by concanavalin A.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4409-13 PMID: 1060120
  20. A calorimetric and fluorescent probe study of the gel-liquid crystalline phase transition in small, single-lamellar dipalmitoylphosphatidylcholine vesicles.
    Biochemistry. 1976 Apr 6;15(7):1393-401 PMID: 1259944
  21. Phospholipid exchange between bilayer membrane vesicles.
    Biochemistry. 1976 Jan 27;15(2):321-7 PMID: 1247519
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.9.4325
Published
1978-09-00
Pages
4325-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336107
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.9.4325 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2