A new and versatile reagent for incorporating multiple primary aliphatic amines into synthetic oligonucleotides.
A novel and versatile phosphoramidite, N-Fmoc-O1-DMT-O2-cyanoethoxydiisopropylamino-phosphinyl-3-am ino-1,2-propanediol (1, Fig. 1), has been synthesized and used to incorporate primary aliphatic amines into synthetic oligonucleotides. Its convenient preparation and use in solid phase oligonucleotide synthesis is described. Using phosphoramidite 1, an amino-modified oligonucleotide probe complementary to M13mp18 DNA was constructed with five primary amines attached to the 5'-terminus. The amino-modified oligonucleotide was subsequently labeled with biotin and employed in a dot-blot hybridization assay. As little as 0.5 ng of M13mp18 target DNA was colorimetrically detected.
15 references, click to expand
-
Chemical synthesis of oligonucleotides containing a free sulphydryl group and subsequent attachment of thiol specific probes.Nucleic Acids Res. 1985 Jun 25;13(12):4485-502 PMID: 4011448
-
The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis.Nucleic Acids Res. 1985 Apr 11;13(7):2399-412 PMID: 4000959
-
Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides.Nucleic Acids Res. 1986 Aug 11;14(15):6227-45 PMID: 3748808
-
The synthesis of oligonucleotides containing a primary amino group at the 5'-terminus.Nucleic Acids Res. 1987 Apr 10;15(7):3131-9 PMID: 3562247
-
Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis.Nucleic Acids Res. 1987 Jun 11;15(11):4593-602 PMID: 3588303
-
The synthesis of protected 5'-mercapto-2',5'-dideoxyribonucleoside-3'-O-phosphoramidites; uses of 5'-mercapto-oligodeoxyribonucleotides.Nucleic Acids Res. 1987 Jun 25;15(12):4837-48 PMID: 3601657
-
Preparation of base-modified nucleosides suitable for non-radioactive label attachment and their incorporation into synthetic oligodeoxyribonucleotides.Nucleic Acids Res. 1987 Jun 25;15(12):4857-76 PMID: 3110740
-
Enzyme-linked synthetic oligonucleotide probes: non-radioactive detection of enterotoxigenic Escherichia coli in faecal specimens.Nucleic Acids Res. 1987 Jul 10;15(13):5275-87 PMID: 3299267
-
The synthesis of protected 5'-amino-2',5'-dideoxyribonucleoside-3'-O-phosphoramidites; applications of 5'-amino-oligodeoxyribonucleotides.Nucleic Acids Res. 1987 Aug 11;15(15):6181-96 PMID: 3627984
-
Synthesis and application of derivatizable oligonucleotides.Nucleic Acids Res. 1987 Aug 25;15(16):6455-67 PMID: 2442728
-
The preparation and application of functionalised synthetic oligonucleotides: III. Use of H-phosphonate derivatives of protected amino-hexanol and mercapto-propanol or -hexanol.Nucleic Acids Res. 1988 Mar 25;16(6):2659-69 PMID: 3362678
-
A modified photometric ninhydrin method for the analysis of amino and imino acids.J Biol Chem. 1953 Feb;200(2):803-11 PMID: 13034841
-
3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate.Gene. 1980 Jul;10(2):177-83 PMID: 6248422
-
Sequence-specific cleavage of single-stranded DNA: oligodeoxynucleotide-EDTA X Fe(II).Proc Natl Acad Sci U S A. 1985 Feb;82(4):968-72 PMID: 3919391
-
Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.Nucleic Acids Res. 1986 Aug 11;14(15):6115-28 PMID: 3748805
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
