Effects of polymorphism (plm) in interleukin (IL) 1 and IL10 genes on surgery outcome in ovarian cancer (OC).

20094 Background: IL1 and IL10 are overexpressed in 55% to 98% of advanced epithelial OC. Different studies demonstrated IL1 and IL10 as independent prognostic factors for OC. This study was performed to investigate the influence of plms of IL-1A, IL-1B and IL-10 gene on the occurrence tumor biology of OC.,In a prospective, monocentric, case-control study 147 pts with OC (71 pts with primary OC and 77 pts with recurrent OC) and 129 pts with no history of malignancies were genotyped for plms of the IL-1 gene (IL-1A -889 T/C and IL-1B -511 C/T promoter) and IL-10 gene (IL-10 -1082 G/A, -819 C/T and -592 C/A). All pts were collected within Tumor bank Ovarian Cancer Project. Genomic DNA fragments were amplified by PCR and the allele sequences were obtained by pyrosequencing. We analyzed significance using the Chi-Square by Pearson and Fisher's exact test. Statistical significance was determined by Mann-Whitney test. A two-tailed p value <0.05 was considered statistically significant. Overall survival (OS) was estimated using the Kaplan-Meier product limited method.,The median age of the pts with OC was 55.5 years (range 19-81 y). Median follow-up period was: 31.5 months (range: 1-225 mo). There were no significant associations between allelic frequencies for IL-1A and IL-1B in OC. The IL-10 plm in -819 and -592 positions significantly correlates with the postoperative residual tumor (p = 0.036 and p = 0.035 respectively). For pts without any postoperative tumor residuals the most encountered genotype was C/C at -819 and -592. The same genotype was associated in univariate analysis with a better OS (95% CI = 37.5-94.5 and 40.8-91.2, respectively). In the multivariate models there was no correlation between any plms and disease-free and OS. Postoperative residual tumor (OR =0.202, 95% CI = 0.088-0.462, p = 0.0001) and ascites volume (OR = 0.106, 95% CI = 0.046-0.248, p = 0.026) were independent prognostic factors for reduced OS.,Plm of IL1A, IL1B, IL10 showed no association with occurrence of OC. IL-10 promoter plm may influence the tumor biology and could potentially predict results of radical surgery. No significant financial relationships to disclose.