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PMID: 278989 Published · ppublish English Journal Article

Transcription patterns of amplified Dytiscus genes coding for ribosomal RNA after injection into Xenopus oocyte nuclei.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 8 ·1978-08-00 ·Pages 3791-5

Trendelenburg MF, Zentgraf H, Franke WW, Gurdon JB

Abstract

Oocytes of the frog Xenopus were injected with purified circular DNA containing amplified rRNA genes of the water beetle Dytiscus. Nuclear contents of injected oocytes were spread and examined by electron microscopy. Most of the Dytiscus DNA seen in injected nuclei contained regions indensely packed with polymerases and nascent transcripts. Apparently normal, as well as abnormal, patterns of transcription were observed. By this type of experiment, it may become possible to recognize the transcribed regions and immediate transcripts of cloned DNA molecules whose activity cannot be seen by electron microscopy of normal nuclei.

MeSH Terms
Animals Cell Nucleus Coleoptera/genetics Female Microscopy, Electron Ovum RNA, Ribosomal/genetics Recombination, Genetic Transcription, Genetic Xenopus/genetics
Chemicals
RNA, Ribosomal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Trendelenburg M F
Zentgraf H
Franke W W
Gurdon J B
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.8.3791
Published
1978-08-00
Pages
3791-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392872
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.8.3791 PMC Full Text View journal details
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