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PMID: 2780285 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Efficient initiation of mammalian mRNA translation at a CUG codon.

Nucleic acids research ·Vol. 17 ·No. 16 ·1989-08-25 ·Pages 6485-97

Dasso MC, Jackson RJ

Abstract

Nucleotide substitutions were made at the initiation codon of an influenza virus NS cDNA clone in a vector carrying the bacteriophage T7 promoter. When capped mRNA transcripts of these constructs were translated in the rabbit reticulocyte lysate, a change in the initiation codon from...AUAAUGG...to...AUACUGG...reduced the in vitro translational efficiency by only 50-60%, and resulted in only a small increase in the yield of short products presumed to be initiated at downstream sites. Synthesis of the full-length product was initiated exclusively at the mutated codon, with negligible use either of in-frame upstream CUG or GUG codons, or of an in-frame downstream GUG codon. We conclude that CUG has the potential to function as an efficient initiation codon in mammalian systems, at least in certain contexts.

MeSH Terms
Animals Base Sequence Cell-Free System Codon/genetics DNA, Viral/genetics Genes, Viral Genetic Vectors Influenza A virus/genetics Molecular Sequence Data Peptide Chain Initiation, Translational Plasmids Promoter Regions, Genetic Protein Biosynthesis RNA, Messenger/genetics Rabbits Restriction Mapping Reticulocytes/metabolism T-Phages/genetics
Chemicals
Codon DNA, Viral RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dasso M C
Department of Biochemistry, University of Cambridge, UK.
Jackson R J
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.16.6485
Published
1989-08-25
Pages
6485-97
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318343
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.16.6485 PMC Full Text View journal details
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