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PMID: 2779559 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Alternative splicing of the platelet-derived growth factor A-chain transcript occurs in normal as well as tumor cells and is conserved among mammalian species.

Molecular and cellular biology ·Vol. 9 ·No. 7 ·1989-07-00 ·Pages 3148-50

Matoskova B, Rorsman F, Svensson V, Betsholtz C

Abstract

Using a polymerase chain reaction approach, we have analyzed the alternative usage of the platelet-derived growth factor A-chain exon 6 in mRNA from various cell types. The results show that this sequence is utilized in a small fraction of the mRNA molecules in normal as well as transformed cells and that this phenomenon is conserved among mammalian species.

MeSH Terms
Animals Biological Evolution Blotting, Southern DNA-Directed DNA Polymerase Exons Fibroblasts/metabolism Gene Amplification Humans Macrophages/metabolism Mammals/genetics Platelet-Derived Growth Factor/genetics RNA Splicing RNA, Messenger/genetics,metabolism Tumor Cells, Cultured
Chemicals
Platelet-Derived Growth Factor RNA, Messenger DNA-Directed DNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Matoskova B
Department of Pathology, University Hospital, Uppsala, Sweden.
Rorsman F
Svensson V
Betsholtz C
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25 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.7.3148-3150.1989
Published
1989-07-00
Pages
3148-50
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362791
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.7.3148-3150.1989 PMC Full Text View journal details
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