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题目:: Regulator of G protein signaling 4 is a novel target of GATA-6 transcription factor.
作者:: Zhang(Yonggang),Li(Fang),Xiao(Xiao),Deng(Wu),Yin(Chaoran),Zhang(Ting),Murthy(Karnam S),Hu(Wenhui)
状态:: 发布时间2016-10-17 , 更新时间 2016-10-28
期刊:: Biochem Biophys Res Commun
摘要:: GATA transcription factors regulate an array of genes important in cell proliferation and differentiation. Here we report the identification of regulator of G protein signaling 4 (RGS4) as a novel target for GATA-6 transcription factor. Although three sites (a, b, c) within the proximal region of rabbit RGS4 promoter for GATA transcription factors were predicted by bioinformatics analysis, only GATA-a site (16 bp from the core TATA box) is essential for RGS4 transcriptional regulation. RT-PCR analysis demonstrated that only GATA-6 was highly expressed in rabbit colonic smooth muscle cells but GATA-4/6 were expressed in cardiac myocytes and GATA-1/2/3 expressed in blood cells. Adenovirus-mediated expression of GATA-6 but not GATA-1 significantly increased the constitutive and IL-1β-induced mRNA expression of the endogenous RGS4 in colonic smooth muscle cells. IL-1β stimulation induced GATA-6 nuclear translocation and increased GATA-6 binding to RGS4 promoter. These data suggest that GATA factor could affect G protein signaling through regulating RGS4 expression, and GATA signaling may develop as a future therapeutic target for RGS4-related diseases.
语言:: eng
DOI:: 10.1016/j.bbrc.2016.10.024

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