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题目:: Spatiotemporal control of cell-cell reversible interactions using molecular engineering.
作者:: Shi(Peng),Ju(Enguo),Yan(Zhengqing),Gao(Nan),Wang(Jiasi),Hou(Jianwen),Zhang(Yan),Ren(Jinsong),Qu(Xiaogang)
状态:: 发布时间2016-10-06 , 更新时间 2016-11-02
期刊:: Nat Commun
摘要:: Manipulation of cell-cell interactions has potential applications in basic research and cell-based therapy. Herein, using a combination of metabolic glycan labelling and bio-orthogonal click reaction, we engineer cell membranes with β-cyclodextrin and subsequently manipulate cell behaviours via photo-responsive host-guest recognition. With this methodology, we demonstrate reversible manipulation of cell assembly and disassembly. The method enables light-controllable reversible assembly of cell-cell adhesion, in contrast with previously reported irreversible effects, in which altered structure could not be reused. We also illustrate the utility of the method by designing a cell-based therapy. Peripheral blood mononuclear cells modified with aptamer are effectively redirected towards target cells, resulting in enhanced cell apoptosis. Our approach allows precise control of reversible cell-cell interactions and we expect that it will promote further developments of cell-based therapy.
语言:: eng
DOI:

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