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PMID: 27694883 Published · epublish English Journal Article Research Support, N.I.H., Extramural

Localized force application reveals mechanically sensitive domains of Piezo1.

Nature communications ·Vol. 7 ·2016-00-03 ·Pages 12939

Wu J, Goyal R, Grandl J

Abstract

Piezos are mechanically activated ion channels that function as sensors of touch and pressure in various cell types. However, the precise mechanism and structures mediating mechanical activation and subsequent inactivation have not yet been identified. Here we use magnetic nanoparticles as localized transducers of mechanical force in combination with pressure-clamp electrophysiology to identify mechanically sensitive domains important for activation and inactivation.

MeSH Terms

Animals Calibration Chickens Electrophysiological Phenomena HEK293 Cells Humans Ion Channels/chemistry Ions Macaca mulatta Magnetics Mechanical Phenomena Mechanotransduction, Cellular Mice Nanoparticles/chemistry Nanotechnology Pressure Protein Domains Rats Signal Transduction Wolves

Chemicals

Ion Channels Ions PIEZO1 protein, human

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Wu Jason

Duke University Medical Center, Department of Neurobiology, Durham, North Carolina 27710, USA.

Goyal Raman

Duke University Medical Center, Department of Neurobiology, Durham, North Carolina 27710, USA.

Grandl Jörg

Duke University Medical Center, Department of Neurobiology, Durham, North Carolina 27710, USA.

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Article Info

Journal

Nature communications

Abbr.

Nat Commun

ISSN

2041-1723

DOI

10.1038/ncomms12939

Published

2016-00-03

Epub

2016-00-03

Pages

12939

Language

English

Region

England

NLM ID

101528555

PMCID

PMC5063965

Subset

Grants

NINDS NIH HHS · F31 NS093777 · United States

NIGMS NIH HHS · T32 GM008441 · United States

External Links

PubMed source DOI: 10.1038/ncomms12939 PMC Full Text View journal details

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