Hybridization of DNA probes with whole-community genome for detection of genes that encode microbial responses to pollutants: mer genes and Hg2+ resistance.

Applied and environmental microbiology ·Vol. 55 ·No. 6 ·1989-06-00 ·Pages 1574-7

Barkay T, Liebert C, Gillman M

Abstract

Nucleic acids extracted from microbial biomass without prior culturing were hybridized with probes representing four mer operons to detect genes encoding adaptation to Hg2+ in whole-community genomes. A 29-fold enrichment in sequences similar to the mer genes of transposon Tn501 occurred during adaptation in a freshwater community. In an estuarine community, all four mer genes were only slightly enriched (by three- to fivefold), suggesting that additional, yet uncharacterized, mer genes encoded adaptation to Hg2+.

MeSH Terms

Adaptation, Physiological Bacteria/drug effects,genetics DNA Probes Drug Resistance, Microbial/genetics Genes, Bacterial Mercury/pharmacology Nucleic Acid Hybridization Operon Water Microbiology Water Pollutants/pharmacology

Chemicals

DNA Probes Water Pollutants Mercury

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Barkay T

Microbial Ecology and Biotechnology Branch U.S., Environmental Protection Agency, Sabine Island, Gulf Breeze, Florida 32561.

Liebert C

Gillman M

References (10)

10 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.55.6.1574-1577.1989

Published

1989-06-00

Pages

1574-7

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC202907

Subset

External Links

PubMed source DOI: 10.1128/aem.55.6.1574-1577.1989 PMC Full Text View journal details

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Hybridization of DNA probes with whole-community genome for detection of genes that encode microbial responses to pollutants: mer genes and Hg2+ resistance.

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