Existence of only a single functional pool of adenosine triphosphate in human erythrocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 6 ·1978-06-00 ·Pages 2825-8

Beutler E, Guinto E, Kuhl W, Matsumoto F

Abstract

The question of whether separate "membrane" and "soluble" pools of ATP exist in erythrocytes has been examined. Phosphoglycerate kinase (EC 2.7.2.3)-derived ("membrane") ATP was labeled by short-term incubation with inorganic [32P]phosphate. Pyruvate kinase (EC 2.7.1.40)-derived ("soluble")ATP is not labeled under these circumstances. The specific activity of the gamma-phosphate of "soluble" ATP was then evaluated by the addition of 2-deoxyglucose and measurement of the specific activity of 2-deoxyglucose-6-[32P]phosphate formed. This specific activity was essentially the same as the overall specific activity of erythrocyte ATP gama-phosphate, indicating that no functional pools of phosphoglycerate kinase-derived and pyruvate kinase-derived ATP exist in erythrocytes.

MeSH Terms

Adenosine Triphosphate/blood Deoxyglucose/blood Diphosphoglyceric Acids/blood Erythrocytes/metabolism Hexokinase/blood Humans Phosphoglycerate Kinase/blood Pyruvate Kinase/blood Solubility

Chemicals

Diphosphoglyceric Acids Adenosine Triphosphate Deoxyglucose Hexokinase Pyruvate Kinase Phosphoglycerate Kinase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Beutler E

Guinto E

Kuhl W

Matsumoto F

References (9)

9 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.6.2825

Published

1978-06-00

Pages

2825-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392657

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.6.2825 PMC Full Text View journal details

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Existence of only a single functional pool of adenosine triphosphate in human erythrocytes.

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