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题目:: Fabrication of type I collagen microcarrier using a microfluidic 3D T-junction device and its application for the quantitative analysis of cell-ECM interactions.
作者:: Yoon(Junghyo),Kim(Jaehoon),Jeong(Hyo Eun),Sudo(Ryo),Park(Myung-Jin),Chung(Seok)
状态:: 发布时间2016-08-26 , 更新时间 2016-08-26
期刊:: Biofabrication
摘要:: We presented a new quantitative analysis for cell and extracellular matrix (ECM) interactions, using cell-coated ECM hydrogel microbeads (hydrobeads) made of type I collagen. The hydrobeads can carry cells as three-dimensional spheroidal forms with an ECM inside, facilitating a direct interaction between the cells and ECM. The cells on hydrobeads do not have a hypoxic core, which opens the possibility for using as a cell microcarrier for bottom-up tissue reconstitution. This technique can utilize various types of cells, even MDA-MB-231 cells, which have weak cell-cell interactions and do not form spheroids in conventional spheroid culture methods. Morphological indices of the cell-coated hydrobead visually present cell-ECM interactions in a quantitative manner.
语言:: eng
DOI:: 10.1088/1758-5090/8/3/035014

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