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题目:: Differentiation potential of rabbit CD90-positive cells sorted from adipose-derived stem cells in vitro.
作者:: Song(Xinghui),Hong(Chaoyang),Zheng(Qingqing),Zhao(Hailan),Song(Kangping),Liu(Zhe),Shen(Jiang),Li(Yanwei),Wang(Jiajia),Shen(Ting)
状态:: 发布时间2016-08-24 , 更新时间 2016-08-24
期刊:: In Vitro Cell Dev Biol Anim
摘要:: To investigate the differentiation potential of purified CD90 cells sorted from adipose-derived stem cells (ADSCs), CD90 cells were sorted from rabbit ADSCs using flow cytometry. Then, cell expansion of CD90 cells and unsorted ADSCs was observed using an inverted microscope. Furthermore, cell surface markers including CD40, CD105, and CD90 on CD90 cells and unsorted ADSCs were quantified using flow cytometry. Additionally, multi-lineage differentiation ability between CD90 cells and unsorted ADSCs was compared, and expression of adipocyte-related genes PPAR-r and CEBPA as well as stem cell-related gene SOX2 in CD90 cells and unsorted ADSCs was determined using real-time quantitative PCR. We found that CD90 cells had a stronger cell proliferation ability than unsorted ADSCs. CD90 cells showed a stronger ability of osteoblast and chondrocyte differentiation than unsorted ADSCs and CD90 cells, whereas the adipose differentiation ability of CD90 cells was similar to that of ADSCs and CD90 cells. CD14, CD105, and CD90 on CD90 cells were expressed more highly than those on ADSCs. Additionally, the mRNA expression level of SOX2 in CD90 cells was significantly higher than that in ADSCs, whereas the expression of PPAR-r and CEBPA was markedly lower than that in ADSCs. These results suggested that the purified CD90 cells sorted from ADSCs exhibit a stronger differentiation potential than the unsorted ADSCs.
语言:: eng
DOI:

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