Home LiteratureArticle Details
PMID: 2754011 Published · ppublish English Journal Article

Capillary gas chromatographic analysis of mycolic acid cleavage products, cellular fatty acids, and alcohols of Mycobacterium xenopi.

Journal of clinical microbiology ·Vol. 27 ·No. 6 ·1989-06-00 ·Pages 1403-6

Luquin M, Lopez F, Ausina V

Abstract

The fatty acids, alcohols, and mycolic acids of 26 strains of Mycobacterium xenopi were studied by capillary gas chromatography and thin-layer chromatography. All strains contained alpha-, keto-, and omega-carboxymycolates. The primary mycolic acid cleavage product was hexacosanoic acid. The fatty acid patterns and, especially, the presence of 2-docosanol are characteristic markers of M. xenopi.

MeSH Terms
Alcohols/analysis Chromatography, Gas Chromatography, Thin Layer Fatty Acids/analysis Mass Spectrometry Mycobacterium/analysis Mycolic Acids/metabolism
Chemicals
Alcohols Fatty Acids Mycolic Acids
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Luquin M
Departamento de Microbiología, Hospital de la Sta., Cruz y San Pablo, Facultad de Medicina de la Universidad, Autónoma de Barcelona, Spain.
Lopez F
Ausina V
References (16)
16 references, click to expand
  1. Gas chromatography of bacterial whole cell methanolysates. I. The usefulness of trimethylsilyl- and trifluoroacetyl derivatives for strain and species characterization.
    Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(5):660-71 PMID: 4344466
  2. Mycobacteria other than Mycobacterium tuberculosis: review of microbiologic and clinical aspects.
    Rev Infect Dis. 1987 Mar-Apr;9(2):275-94 PMID: 3296098
  3. Identification of clinical isolates of mycobacteria with gas-liquid chromatography alone.
    J Clin Microbiol. 1979 Oct;10(4):506-14 PMID: 118984
  4. Clinical and roentgenographic features of nosocomial pulmonary disease due to Mycobacterium xenopi.
    Am Rev Respir Dis. 1981 Jan;123(1):104-9 PMID: 7458072
  5. Gas-chromatographic analysis of mycolic acid cleavage products in mycobacteria.
    J Clin Microbiol. 1981 May;13(5):899-907 PMID: 7240402
  6. A review of the methods of identification and differentiation of mycobacteria.
    Rev Infect Dis. 1981 Sep-Oct;3(5):841-61 PMID: 7041220
  7. Lipid analysis for the identification of mycobacteria: an appraisal.
    Rev Infect Dis. 1981 Sep-Oct;3(5):862-6 PMID: 7339816
  8. Determination of fatty acids and carbohydrate monomers in micro-organisms by means of glass capillary gas chromatography: analysis of Mycobacterium gordonae and Mycobacterium scrofulaceum.
    J Gen Microbiol. 1983 Feb;129(2):401-5 PMID: 6842180
  9. Clinical review of pulmonary disease caused by Mycobacterium xenopi.
    Thorax. 1983 May;38(5):373-7 PMID: 6879487
  10. [Taxonomic value of mycobacterial fatty acids: proposal for a method of analysis].
    Ann Microbiol (Paris). 1983 Sep-Oct;134B(2):241-56 PMID: 6651121
  11. Mycobacterium xenopi infection in a patient with acquired immunodeficiency syndrome.
    Chest. 1984 Jul;86(1):145-7 PMID: 6734278
  12. Mycolic acid patterns of some species of Mycobacterium.
    Arch Microbiol. 1984 Oct;139(2-3):225-31 PMID: 6517656
  13. Rapid identification of mycobacteria using gas liquid chromatography.
    Pathology. 1985 Jan;17(1):24-8 PMID: 4000712
  14. Gas chromatographic fatty acid profiles for characterisation of mycobacteria: an interlaboratory methodological evaluation.
    Eur J Clin Microbiol. 1985 Oct;4(5):483-7 PMID: 4065134
  15. Analysis of mycolic acid cleavage products and cellular fatty acids of Mycobacterium species by capillary gas chromatography.
    J Clin Microbiol. 1986 Apr;23(4):731-6 PMID: 3084554
  16. Nontuberculous mycobacteria and associated diseases.
    Am Rev Respir Dis. 1979 Jan;119(1):107-59 PMID: 369415
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
Published
1989-06-00
Pages
1403-6
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC267571
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com