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题目:: SR proteins regulate V6 exon splicing of CD44 pre-mRNA.
作者:: Loh(Tiing Jen),Moon(Heegyum),Jang(Ha Na),Liu(Yongchao),Choi(Namjeong),Shen(Shengfu),Williams(Darren Reece),Jung(Da-Woon),Zheng(Xuexiu),Shen(Haihong)
状态:: 发布时间2016-08-17 , 更新时间 2016-11-24
期刊:: BMB Rep
摘要:: CD44 pre-mRNA includes 20 exons, of which exons 1-5 (C-C) and exons 16-20 (C-C) are constant exons, whereas exons 6-15 (V-V) are variant exons. V-exon-containing isoforms have been known to be implicated in tumor cell invasion and metastasis. In the present study, we performed a SR protein screen for CD44 V splicing using overexpression and lentivirus-mediated shRNA treatment. Using a CD44 V minigene, we demonstrate that increased SRSF3 and SRSF4 expression do not affect V splicing, but increased expression of SRSF1, SRSF6 and SRSF9 significantly inhibit V splicing. In addition, using a constitutive exon-specific primer set, we could not detect alterations of CD44 splicing after SR protein-targeting shRNA treatment. However, using a V specific primer, we identified that reduced SRSF2 expression significantly reduced the V isoform, but increased V and V isoforms. Our results indicate that SR proteins are important regulatory proteins for CD44 V splicing. [BMB Reports 2016; 49(11): 612-616].
语言:: eng
DOI:

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