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PMID: 2747623 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cotranscription of the S10- and spc-like operons in spinach chloroplasts and identification of three of their gene products.

Molecular & general genetics : MGG ·Vol. 216 ·No. 2-3 ·1989-04-00 ·Pages 439-45

Zhou DX, Quigley F, Massenet O, Mache R

Abstract

The organisation and expression of the rpl22, rps3, rpl16 and rpl14 genes, which belong to the S10- and spc-like operons of spinach chloroplasts, have been studied. Northern experiments and nuclease S1 mapping show that the two operon-like groups of genes are cotranscribed. It is demonstrated that the intron-containing rpl16 gene is spliced in vivo. Based on amino acid composition and protein sequence data, the products of the rpl22, rpl16 and rpl14 genes are identified respectively as the spinach chloroplast ribosomal proteins CS-L13, CS-L24 and CS-L29. The rpl22 gene product is a 5S rRNA binding protein and therefore is distinguishable from the homologous Escherichia coli L22 ribosomal protein.

MeSH Terms
Amino Acid Sequence Base Sequence Chloroplasts/metabolism DNA/genetics DNA, Recombinant Molecular Sequence Data Operon Plants/genetics Ribosomal Proteins/genetics Transcription, Genetic
Chemicals
DNA, Recombinant Ribosomal Proteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zhou D X
Centre National de la Recherche Scientifique (URA 57), Université Joseph Fourier, Grenoble, France.
Quigley F
Massenet O
Mache R
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18 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00334388
Published
1989-04-00
Pages
439-45
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X13336
External Links
PubMed source DOI: 10.1007/BF00334388
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