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PMID: 2746732 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interplay between carbohydrate in the stalk and the length of the connecting peptide determines the cleavability of influenza virus hemagglutinin.

Journal of virology ·Vol. 63 ·No. 8 ·1989-08-00 ·Pages 3296-300

Kawaoka Y, Webster RG

Abstract

The ability of many viruses to replicate in host cells depends on cleavage of certain viral glycoproteins, including hemagglutinin (HA). By generating site-specific mutant HAs of two highly virulent influenza viruses, we established that the relationship between carbohydrate in the stalk and the length of the connecting peptide is a critical determinant of cleavability. HAs that lacked an oligosaccharide side chain in the stalk were cleaved regardless of the number of basic amino acids at the cleavage site, whereas those with the oligosaccharide side chain resisted cleavage unless additional basic amino acids were inserted. This finding suggests that the oligosaccharide side chain interferes with HA cleavage if the number of basic amino acids at the cleavage site is not adequate to nullify this effect. Similar interplay could influence cleavage of other viral glycoproteins, such as those of human and simian immunodeficiency viruses and paramyxoviruses.

MeSH Terms
Amino Acid Sequence Amino Acids Animals Carbohydrate Metabolism Glycosylation Hemagglutinins, Viral/genetics,metabolism Influenza A virus/genetics,metabolism Molecular Sequence Data Mutation Oligosaccharides/metabolism
Chemicals
Amino Acids Hemagglutinins, Viral Oligosaccharides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kawaoka Y
Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Webster R G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.8.3296-3300.1989
Published
1989-08-00
Pages
3296-300
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250901
Subset
IM
Grants
NIAID NIH HHS · AI 52586 · United States
NCI NIH HHS · CA 21765 · United States
External Links
PubMed source DOI: 10.1128/JVI.63.8.3296-3300.1989 PMC Full Text View journal details
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