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PMID: 2738096 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Differential structural requirements for fibrinogen binding to platelets and to endothelial cells.

The Journal of cell biology ·Vol. 108 ·No. 6 ·1989-06-00 ·Pages 2519-27

Tranqui L, Andrieux A, Hudry-Clergeon G, Ryckewaert JJ, Soyez S, Chapel A, Ginsberg MH, Plow EF, Marguerie G

Abstract

The cytoadhesins represent a group of RGD receptors that belongs to the integrin superfamily of adhesion molecules. Members of this cytoadhesin family include the platelet GPIIb-IIIa and the vitronectin receptors. These glycoproteins share the same beta-subunit, which is associated with different alpha subunits to form an alpha/beta heterodimer. In the present study, we have analyzed the fine recognition specificy of the cytoadhesins from platelets and endothelial cells for the adhesive protein, fibrinogen. Two sets of synthetic peptides, RGDX peptides and peptides corresponding to the COOH terminus of the fibrinogen gamma chain, were compared for their structure-function relationships in the two cellular systems. The results indicate that: (a) both RGDX and gamma-chain peptides inhibit the binding of fibrinogen to platelets and endothelial cells; (b) a marked influence of the residue at the COOH- and NH2-terminal positions of each peptide set can be demonstrated on the two types; and (c) RGDX and gamma peptides have differential effects on platelets and endothelial cells with respect to fine structural requirements. These results clearly indicate that while the platelet and endothelial cytoadhesins may interact with similar peptidic sequences, they express a different fine structural recognition.

MeSH Terms
Actin Cytoskeleton/ultrastructure Amino Acid Sequence Binding, Competitive Blood Platelets/metabolism Cell Adhesion Endothelium, Vascular/metabolism Fibrinogen/metabolism Humans In Vitro Techniques Oligopeptides/metabolism Platelet Aggregation Platelet Membrane Glycoproteins/metabolism Solubility Structure-Activity Relationship
Chemicals
Oligopeptides Platelet Membrane Glycoproteins Fibrinogen
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Tranqui L
DRF/Laboratoire d'Hématologie, Unité INSERM 217, Grenoble, France.
Andrieux A
Hudry-Clergeon G
Ryckewaert J J
Soyez S
Chapel A
Ginsberg M H
Plow E F
Marguerie G
References (48)
48 references, click to expand
  1. The amino acid sequence of the alpha-chain of human fibrinogen.
    Nature. 1979 Aug 9;280(5722):464-8 PMID: 460425
  2. An Arg-Gly-Asp-directed receptor on the surface of human melanoma cells exists in an divalent cation-dependent functional complex with the disialoganglioside GD2.
    J Cell Biol. 1987 Sep;105(3):1163-73 PMID: 2443507
  3. Desmin and vimentin coexist at the periphery of the myofibril Z disc.
    Cell. 1979 Dec;18(4):1053-63 PMID: 391403
  4. Prediction of protein antigenic determinants from amino acid sequences.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824-8 PMID: 6167991
  5. Specific and saturable binding of plasma fibronectin to thrombin-stimulated human platelets.
    J Biol Chem. 1981 Sep 25;256(18):9477-82 PMID: 6270087
  6. The platelet glycoprotein IIb/IIIa-like protein in human endothelial cells promotes adhesion but not initial attachment to extracellular matrix.
    J Cell Biol. 1987 Oct;105(4):1885-92 PMID: 2822728
  7. Influence of stereochemistry of the sequence Arg-Gly-Asp-Xaa on binding specificity in cell adhesion.
    J Biol Chem. 1987 Dec 25;262(36):17294-8 PMID: 3693352
  8. Chemical cross-linking of arginyl-glycyl-aspartic acid peptides to an adhesion receptor on platelets.
    J Biol Chem. 1988 Mar 15;263(8):3943-51 PMID: 3346230
  9. Cytoadhesins, integrins, and platelets.
    Thromb Haemost. 1988 Feb 25;59(1):1-6 PMID: 3283991
  10. SOLID-PHASE PEPTIDE SYNTHESIS. 3. AN IMPROVED SYNTHESIS OF BRADYKININ.
    Biochemistry. 1964 Sep;3:1385-90 PMID: 14229685
  11. Serial propagation of human endothelial cells in vitro.
    J Cell Biol. 1981 Nov;91(2 Pt 1):420-6 PMID: 7309790
  12. Adenosine diphosphate induces binding of von Willebrand factor to human platelets.
    Nature. 1982 May 13;297(5862):154-6 PMID: 6281653
  13. gamma and alpha chains of human fibrinogen possess sites reactive with human platelet receptors.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):2068-71 PMID: 6281794
  14. The cell attachment domain of fibronectin. Determination of the primary structure.
    J Biol Chem. 1982 Aug 25;257(16):9593-7 PMID: 7050098
  15. Glanzmann thrombasthenia: deficient binding of von Willebrand factor to thrombin-stimulated platelets.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):6038-41 PMID: 6310559
  16. Evidence that three adhesive proteins interact with a common recognition site on activated platelets.
    J Biol Chem. 1984 May 10;259(9):5388-91 PMID: 6325435
  17. Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule.
    Nature. 1984 May 3-9;309(5963):30-3 PMID: 6325925
  18. Platelet receptor recognition site on human fibrinogen. Synthesis and structure-function relationship of peptides corresponding to the carboxy-terminal segment of the gamma chain.
    Biochemistry. 1984 Apr 10;23(8):1767-74 PMID: 6326808
  19. Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.
    Proc Natl Acad Sci U S A. 1966 Jan;55(1):134-41 PMID: 5220862
  20. Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides.
    Anal Biochem. 1970 Apr;34(2):595-8 PMID: 5443684
  21. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria.
    J Clin Invest. 1973 Nov;52(11):2745-56 PMID: 4355998
  22. Binding of soluble form of fibroblast surface protein, fibronectin, to collagen.
    Int J Cancer. 1977 Jul 15;20(1):1-5 PMID: 903179
  23. Human platelets possess an inducible and saturable receptor specific for fibrinogen.
    J Biol Chem. 1979 Jun 25;254(12):5357-63 PMID: 447655
  24. Interaction between fibrinogen and cultured endothelial cells. Induction of migration and specific binding.
    J Clin Invest. 1985 Jan;75(1):11-8 PMID: 3965498
  25. Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor.
    Cell. 1985 Jan;40(1):191-8 PMID: 3155652
  26. Interaction of fibronectin with its receptor on platelets.
    Cell. 1985 Sep;42(2):439-48 PMID: 2411420
  27. Inhibition of platelet adhesion to fibronectin, fibrinogen, and von Willebrand factor substrates by a synthetic tetrapeptide derived from the cell-binding domain of fibronectin.
    Blood. 1985 Oct;66(4):946-52 PMID: 3876125
  28. The tetrapeptide analogue of the cell attachment site of fibronectin inhibits platelet aggregation and fibrinogen binding to activated platelets.
    J Biol Chem. 1985 Oct 5;260(22):11891-4 PMID: 2995350
  29. The effect of Arg-Gly-Asp-containing peptides on fibrinogen and von Willebrand factor binding to platelets.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):8057-61 PMID: 3877935
  30. Arg-Gly-Asp: a versatile cell recognition signal.
    Cell. 1986 Feb 28;44(4):517-8 PMID: 2418980
  31. Platelet membrane glycoprotein IIb/IIIa: member of a family of Arg-Gly-Asp--specific adhesion receptors.
    Science. 1986 Mar 28;231(4745):1559-62 PMID: 2420006
  32. Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin.
    Cell. 1986 Jul 18;46(2):271-82 PMID: 3487386
  33. Immunologic relationship between platelet membrane glycoprotein GPIIb/IIIa and cell surface molecules expressed by a variety of cells.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):6002-6 PMID: 2426709
  34. Evidence that arginyl-glycyl-aspartate peptides and fibrinogen gamma chain peptides share a common binding site on platelets.
    J Biol Chem. 1987 Jan 25;262(3):947-50 PMID: 3805026
  35. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  36. Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family.
    Cell. 1987 Feb 27;48(4):681-90 PMID: 3028646
  37. Competition for related but nonidentical binding sites on the glycoprotein IIb-IIIa complex by peptides derived from platelet adhesive proteins.
    Cell. 1987 Mar 13;48(5):867-73 PMID: 3493077
  38. The VLA protein family. Characterization of five distinct cell surface heterodimers each with a common 130,000 molecular weight beta subunit.
    J Biol Chem. 1987 Mar 5;262(7):3300-9 PMID: 3546305
  39. Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone. Identity with platelet glycoprotein IIIa and similarity to "integrin".
    J Biol Chem. 1987 Mar 25;262(9):3936-9 PMID: 3494014
  40. Fibronectin receptor structures in the VLA family of heterodimers.
    Nature. 1987 Apr 9-15;326(6113):607-9 PMID: 3031508
  41. C3bi receptor (complement receptor type 3) recognizes a region of complement protein C3 containing the sequence Arg-Gly-Asp.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1965-8 PMID: 3550803
  42. Immunochemical and amino-terminal sequence comparison of two cytoadhesins indicates they contain similar or identical beta subunits and distinct alpha subunits.
    J Biol Chem. 1987 Apr 25;262(12):5437-40 PMID: 2437107
  43. Fibrinogen induces adhesion, spreading, and microfilament organization of human endothelial cells in vitro.
    J Cell Biol. 1987 May;104(5):1403-11 PMID: 2437130
  44. Structure of the platelet membrane glycoprotein IIb. Homology to the alpha subunits of the vitronectin and fibronectin membrane receptors.
    J Biol Chem. 1987 Jun 25;262(18):8476-82 PMID: 2439501
  45. Platelet glycoprotein IIb-IIIa-like proteins mediate endothelial cell attachment to adhesive proteins and the extracellular matrix.
    J Biol Chem. 1987 Jul 25;262(21):9935-8 PMID: 2440865
  46. Human endothelial cells synthesize and express an Arg-Gly-Asp-directed adhesion receptor involved in attachment to fibrinogen and von Willebrand factor.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6471-5 PMID: 2442758
  47. Amino acid sequence of the vitronectin receptor alpha subunit and comparative expression of adhesion receptor mRNAs.
    J Biol Chem. 1987 Oct 15;262(29):14080-5 PMID: 2443500
  48. Exposure of platelet fibrinogen receptors by ADP and epinephrine.
    J Clin Invest. 1979 Nov;64(5):1393-401 PMID: 574143
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.108.6.2519
Published
1989-06-00
Pages
2519-27
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115601
Subset
IM
Grants
NHLBI NIH HHS · HL 38292 · United States
External Links
PubMed source DOI: 10.1083/jcb.108.6.2519 PMC Full Text View journal details
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