Abstract
The Semliki Forest virus capsid (C) protein was introduced into various target cells by electroporation-, liposome-, and erythrocyte-ghost-mediated delivery. Data are presented which show that the incorporated C protein is biologically active and, at low concentrations (10(3) to 10(4) molecules per cell), markedly induces host cellular protein synthesis (average value, up to 90%). On the other hand, high concentrations (10(5) to 10(6) molecules per cell) led to a significant inhibition (average value, up to 60%). The cellular response to C protein was found to be identical in P3X63Ag8 suspension cells, CV-1 cells, and GpBind4 cells. Following electroporation-mediated delivery of C-protein molecules, both induction and repression of cellular protein synthesis were immediate, whereas with liposome-mediated delivery these events were delayed by about 1 h. Maximum stimulation and repression occurred between 0 and 1 h after delivery of C protein and decreased thereafter to reach control values at about 4 h. The analysis of the proteins synthesized suggests that low amounts of microinjected C protein are responsible for the induction of classes with specific Mrs, whereas high amounts lead to an inhibition of overall protein synthesis.
MeSH Terms
Animals
Capsid/isolation & purification,physiology
Cell Line
Cell Transformation, Viral
Erythrocyte Membrane/metabolism
Genes, Regulator
Kinetics
Liposomes
Molecular Weight
Neoplasm Proteins/biosynthesis
Protein Biosynthesis
Semliki forest virus/genetics
Chemicals
Liposomes
Neoplasm Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Elgizoli M
Institute of Hygiene and Medical Microbiology, University of Berne, Switzerland.
Dai Y
Kempf C
Koblet H
Michel M R
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