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PMID: 27231092 Published · ppublish English Journal Article

Shot and Patronin polarise microtubules to direct membrane traffic and biogenesis of microvilli in epithelia.

Journal of cell science ·Vol. 129 ·No. 13 ·2016-00-01 ·Pages 2651-9

Khanal I, Elbediwy A, Diaz de la Loza Mdel C, Fletcher GC, Thompson BJ

Abstract

In epithelial tissues, polarisation of microtubules and actin microvilli occurs along the apical-basal axis of each cell, yet how these cytoskeletal polarisation events are coordinated remains unclear. Here, we examine the hierarchy of events during cytoskeletal polarisation in Drosophila melanogaster epithelia. Core apical-basal polarity determinants polarise the spectrin cytoskeleton to recruit the microtubule-binding proteins Patronin (CAMSAP1, CAMSAP2 and CAMSAP3 in humans) and Shortstop [Shot; MACF1 and BPAG1 (also known as DST) in humans] to the apical membrane domain. Patronin and Shot then act to polarise microtubules along the apical-basal axis to enable apical transport of Rab11 endosomes by the Nuf-Dynein microtubule motor complex. Finally, Rab11 endosomes are transferred to the MyoV (also known as Didum in Drosophila) actin motor to deliver the key microvillar determinant Cadherin 99C to the apical membrane to organise the biogenesis of actin microvilli.

Keywords
Drosophila Epithelia Microtubules Microvilli Polarity Spectrin
MeSH Terms
Actin Cytoskeleton/genetics,metabolism Animals Cadherins/metabolism Cell Membrane/genetics,metabolism Cell Polarity/genetics Drosophila Proteins/genetics,metabolism Drosophila melanogaster/genetics Epithelium/growth & development,metabolism Humans Microfilament Proteins/genetics Microtubule-Associated Proteins/genetics Microtubules/genetics Microvilli/genetics,metabolism Myosin Type V/genetics,metabolism Protein Transport/genetics rab GTP-Binding Proteins/genetics,metabolism
Chemicals
Cadherins Drosophila Proteins Microfilament Proteins Microtubule-Associated Proteins didum protein, Drosophila patronin protein, Drosophila shot protein, Drosophila Myosin Type V Rab11 protein, Drosophila rab GTP-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Khanal Ichha
The Francis Crick Institute, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
Elbediwy Ahmed
The Francis Crick Institute, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
Diaz de la Loza Maria Del Carmen
The Francis Crick Institute, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
Fletcher Georgina C
The Francis Crick Institute, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
Thompson Barry J ORCID
The Francis Crick Institute, 44 Lincoln's Inn Fields, London WC2A 3LY, UK barry.thompson@crick.ac.uk.
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Article Info
Journal
Journal of cell science
Abbr.
J Cell Sci
ISSN
1477-9137
DOI
10.1242/jcs.189076
Email
barry.thompson@crick.ac.uk
Published
2016-00-01
Epub
2016-00-26
Pages
2651-9
Language
English
Region
England
NLM ID
0052457
PMCID
PMC4958304
Subset
IM
Grants
Wellcome Trust · United Kingdom
Wellcome Trust · 102853/B/13/Z · United Kingdom
Corrections
ErratumIn
PMID: 28668933
External Links
PubMed source DOI: 10.1242/jcs.189076 PMC Full Text View journal details
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