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题目:: Cytokine-induced killer cells interact with tumor lysate-pulsed dendritic cells via CCR5 signaling.
作者:: Lee(Hong Kyung),Kim(Yong Guk),Kim(Ji Sung),Park(Eun Jae),Kim(Boyeong),Park(Ki Hwan),Kang(Jong Soon),Hong(Jin Tae),Kim(Youngsoo),Han(Sang-Bae)
状态:: 发布时间2016-06-06 , 更新时间 2016-06-06
期刊:: Cancer Lett
摘要:: The antitumor activity of cytokine-induced killer (CIK) cells can be increased by co-culturing them with tumor lysate-pulsed dendritic cells (tDCs); this phenomenon has been studied mainly at the population level. Using time-lapse imaging, we examined how CIK cells gather information from tDCs at the single-cell level. tDCs highly expressed CCL5, which bound CCR5 expressed on CIK cells. tDCs strongly induced migration of Ccr5(+/+) CIK cells, but not that of Ccr5(-/-) CIK cells or Ccr5(+/+) CIK cells treated with the CCR5 antagonist Maraviroc. Individual tDCs contacted Ccr5(+/+) CIK cells more frequently and lengthily than with Ccr5(-/-) CIK cells. Consequently, tDCs increased the antitumor activity of Ccr5(+/+) CIK cells in vitro and in vivo, but did not increase that of Ccr5(-/-) CIK cells. Taken together, our data provide insight into the mechanism of CIK cell activation by tDCs at the single-cell level.
语言:: eng
DOI:: 10.1016/j.canlet.2016.05.020

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