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PMID: 271970 Published · ppublish English Journal Article

Two distinct adenosine-sensitive sites on adenylate cyclase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 12 ·1977-12-00 ·Pages 5482-6

Londos C, Wolff J

Abstract

The effects of adenosine and adenosine analogs on adenylate cyclases from several tissues have been examined. Two adenosine-reactive sites have been identified: (i) the "R" site, occupancy of which usually leads to activation of cyclase and which requires integrity of the ribose ring for activity, and (ii) the "P" site, which mediates inhibition and requires integrity of the purine ring for activity. Biphasic effects of adenosine are explained by the presence of both sites on a single adenylate cyclase. Comparison of these data with those in the literature indicates that adenosine-reactive "P" and "R" sites are present generally.

MeSH Terms
Adenosine/analogs & derivatives,pharmacology Adenylyl Cyclase Inhibitors Adenylyl Cyclases/metabolism Adrenal Glands/enzymology Allosteric Site Animals Blood Platelets/enzymology Cattle Enzyme Activation Humans Kinetics Leydig Cells/enzymology Liver/enzymology Male Mice Rats
Chemicals
Adenylyl Cyclase Inhibitors Adenylyl Cyclases Adenosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Londos C
Wolff J
References (32)
32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.12.5482
Published
1977-12-00
Pages
5482-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431772
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.12.5482 PMC Full Text View journal details
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