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PMID: 2715180 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The expression of sarcomeric muscle-specific contractile protein genes in BC3H1 cells: BC3H1 cells resemble skeletal myoblasts that are defective for commitment to terminal differentiation.

The Journal of cell biology ·Vol. 108 ·No. 5 ·1989-05-00 ·Pages 1799-806

Taubman MB, Smith CW, Izumo S, Grant JW, Endo T, Andreadis A, Nadal-Ginard B

Abstract

The BC3H1 cell line has been used widely as a model for studying regulation of muscle-related proteins, such as the acetylcholine receptor, myokinase, creatine kinase, and actin. These cells, derived from a nitrosourea-induced mouse brain neoplasm, have some of the morphological characteristics of smooth muscle and have been shown to express the vascular smooth muscle isoform of alpha-actin. To provide further information about the contractile protein phenotype of BC3H1 and to gain additional insights into the possible tissue of origin of these cells, we have examined the expression of a battery of contractile protein genes. During rapid growth, subconfluent BC3H1 cells express the nonmuscle isoform of alpha-tropomyosin (alpha-Tm) and the nonsarcomeric isoforms of myosin heavy and light chains (MHCs and MLCs, respectively), but do not express troponin T(TnT). However, when BC3H1 cells differentiate in response to incubation in serum-deprived medium or upon approaching confluence, they express TnT as well as sarcomeric muscle isoforms of MHC, MLC 2 and 3, alpha-Tm, and alpha-actin. These results suggest that BC3H1 is a skeletal muscle cell line of ectodermal origin that is defective for commitment to terminal differentiation.

MeSH Terms
Actins/genetics Animals Cell Differentiation Cell Line DNA/genetics DNA Probes Genes Mice Muscle Proteins/genetics Muscles/cytology,metabolism Myofibrils/metabolism Myosins/genetics Nucleic Acid Hybridization Phenotype RNA, Messenger/genetics Sarcomeres/metabolism Transcription, Genetic
Chemicals
Actins DNA Probes Muscle Proteins RNA, Messenger DNA Myosins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Taubman M B
Department of Cardiology, Children's Hospital, Boston, MA.
Smith C W
Izumo S
Grant J W
Endo T
Andreadis A
Nadal-Ginard B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.108.5.1799
Published
1989-05-00
Pages
1799-806
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115545
Subset
IM
Grants
NHLBI NIH HHS · HL01724 · United States
NHLBI NIH HHS · HL07101 · United States
External Links
PubMed source DOI: 10.1083/jcb.108.5.1799 PMC Full Text View journal details
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