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PMID: 2708325 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein.

Journal of bacteriology ·Vol. 171 ·No. 5 ·1989-05-00 ·Pages 2882-5

Ubukata K, Nonoguchi R, Matsuhashi M, Konno M

Abstract

A beta-lactam-sensitive strain of Staphylococcus aureus could be converted to methicillin resistance by the introduction of a plasmid carrying the 4.3-kilobase HindIII chromosomal DNA fragment which encoded the mecA gene from a methicillin-resistant S. aureus. Transformant cells produced methicillin-resistant S. aureus-specific penicillin-binding protein constitutively, and additional insertion of an inducible penicillinase plasmid caused production of the pencillin-binding protein to become inducible.

MeSH Terms
Bacterial Proteins Carrier Proteins/genetics Cloning, Molecular Gene Expression Regulation Hexosyltransferases Muramoylpentapeptide Carboxypeptidase/genetics Penicillin Resistance Penicillin-Binding Proteins Peptidyl Transferases Staphylococcus aureus/genetics
Chemicals
Bacterial Proteins Carrier Proteins Penicillin-Binding Proteins Peptidyl Transferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ubukata K
Department of Clinical Pathology, Teikyo University School of Medicine, Tokyo, Japan.
Nonoguchi R
Matsuhashi M
Konno M
References (17)
17 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.5.2882-2885.1989
Published
1989-05-00
Pages
2882-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209980
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.5.2882-2885.1989 PMC Full Text View journal details
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