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PMID: 2707851 Published · ppublish English Journal Article

Expression of pertussis toxin in Bordetella bronchiseptica and Bordetella parapertussis carrying recombinant plasmids.

Infection and immunity ·Vol. 57 ·No. 5 ·1989-05-00 ·Pages 1413-8

Lee CK, Roberts A, Perrin S

Abstract

Pertussis toxin is produced only by strains of Bordetella pertussis. Cloned genes encoding pertussis toxin from B. pertussis were transferred into Bordetella bronchiseptica and Bordetella parapertussis by conjugation. These transconjugants expressed pertussis toxin at levels comparable to those expressed by B. pertussis. The toxin made by these strains was biologically active in the Chinese hamster cell clumping assay, contained all five subunits, and was mostly periplasmic. Toxin expression appeared to be modulated in the same way as are the vir-regulated genes of B. pertussis. Introduction of these plasmids into B. pertussis failed to produce hypertoxigenic strains. Instead, these transconjugants underwent plasmid loss, gene deletions, or conversion to the avirulent phase.

MeSH Terms
Antibodies, Monoclonal/immunology Bordetella/genetics Cloning, Molecular Conjugation, Genetic Gene Expression Regulation Molecular Weight Pertussis Toxin Pertussis Vaccine/genetics Plasmids Recombinant Proteins Vaccines, Synthetic Virulence Factors, Bordetella/genetics,immunology
Chemicals
Antibodies, Monoclonal Pertussis Vaccine Recombinant Proteins Vaccines, Synthetic Virulence Factors, Bordetella Pertussis Toxin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee C K
Biologic Laboratories, Center for Disease Control of the Massachusetts Department of Public Health, Boston 02130.
Roberts A
Perrin S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.5.1413-1418.1989
Published
1989-05-00
Pages
1413-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313292
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.57.5.1413-1418.1989 PMC Full Text View journal details
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