Home LiteratureArticle Details
PMID: 2694960 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes.

Applied and environmental microbiology ·Vol. 55 ·No. 12 ·1989-12-00 ·Pages 3162-6

Zylstra GJ, Wackett LP, Gibson DT

Abstract

Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroethylene degradation by the recombinant organism depended on the cell concentration and the concentration of trichloroethylene. A linear rate of trichloroethylene degradation was observed with the E. coli recombinant strain. In contrast, P. putida F39/D, a mutant strain of P. putida F1 that does not contain cis-toluene dihydrodiol dehydrogenase, showed a much faster initial rate of trichloroethylene degradation which decreased over time.

MeSH Terms
Biodegradation, Environmental Chemical Phenomena Chemistry Chromatography, Gas Cloning, Molecular Escherichia coli/enzymology,genetics Oxidation-Reduction Oxygenases/genetics,metabolism Pseudomonas/enzymology,genetics Trichloroethylene/metabolism
Chemicals
Trichloroethylene Oxygenases toluene dioxygenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zylstra G J
Department of Microbiology, University of Iowa, Iowa City 52242.
Wackett L P
Gibson D T
References (23)
23 references, click to expand
  1. Trichloroethylene biodegradation by a methane-oxidizing bacterium.
    Appl Environ Microbiol. 1988 Apr;54(4):951-6 PMID: 16347616
  2. Toluene dioxygenase: a multicomponent enzyme system.
    Biochem Biophys Res Commun. 1977 Sep 9;78(1):401-10 PMID: 907688
  3. Toxicity of Trichloroethylene to Pseudomonas putida F1 Is Mediated by Toluene Dioxygenase.
    Appl Environ Microbiol. 1989 Oct;55(10):2723-5 PMID: 16348039
  4. Metabolism of volatile chlorinated aliphatic hydrocarbons by Pseudomonas fluorescens.
    Appl Environ Microbiol. 1988 Oct;54(10):2578-9 PMID: 3144246
  5. Purification and properties of ferredoxinTOL. A component of toluene dioxygenase from Pseudomonas putida F1.
    J Biol Chem. 1985 Feb 25;260(4):2355-63 PMID: 2982815
  6. Trichloroethylene metabolism by microorganisms that degrade aromatic compounds.
    Appl Environ Microbiol. 1988 Feb;54(2):604-6 PMID: 3355147
  7. THE DECOMPOSITION OF TOLUENE BY SOIL BACTERIA.
    J Gen Microbiol. 1964 Jul;36:107-22 PMID: 14192532
  8. Purification and properties of NADH-ferredoxinTOL reductase. A component of toluene dioxygenase from Pseudomonas putida.
    J Biol Chem. 1981 Mar 25;256(6):2723-30 PMID: 7204373
  9. Degradation of trichloroethylene by the ammonia-oxidizing bacterium Nitrosomonas europaea.
    Biochem Biophys Res Commun. 1989 Mar 15;159(2):640-3 PMID: 2930535
  10. Regulation of ribosomal RNA promoters with a synthetic lac operator.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6929-33 PMID: 6390428
  11. Oxidation of trichloroethylene by liver microsomal cytochrome P-450: evidence for chlorine migration in a transition state not involving trichloroethylene oxide.
    Biochemistry. 1982 Mar 2;21(5):1090-7 PMID: 7074051
  12. The aerobic pseudomonads: a taxonomic study.
    J Gen Microbiol. 1966 May;43(2):159-271 PMID: 5963505
  13. Biodegradation of chlorinated ethenes by a methane-utilizing mixed culture.
    Appl Environ Microbiol. 1986 Apr;51(4):720-4 PMID: 3085587
  14. Biodegradation of trichloroethylene and involvement of an aromatic biodegradative pathway.
    Appl Environ Microbiol. 1987 May;53(5):949-54 PMID: 3606099
  15. Formation of (+)-cis-2,3-dihydroxy-1-methylcyclohexa-4,6-diene from toluene by Pseudomonas putida.
    Biochemistry. 1970 Mar 31;9(7):1626-30 PMID: 4314232
  16. Aerobic metabolism of trichloroethylene by a bacterial isolate.
    Appl Environ Microbiol. 1986 Aug;52(2):383-4 PMID: 16347139
  17. Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography.
    Biochem Biophys Res Commun. 1979 Dec 14;91(3):1131-9 PMID: 526270
  18. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  19. Novel pathway of toluene catabolism in the trichloroethylene-degrading bacterium g4.
    Appl Environ Microbiol. 1989 Jun;55(6):1624-9 PMID: 16347956
  20. Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli.
    J Biol Chem. 1989 Sep 5;264(25):14940-6 PMID: 2670929
  21. Degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with Pseudomonas putida F1.
    Appl Environ Microbiol. 1988 Jul;54(7):1703-8 PMID: 3415234
  22. Biotransformation of tetrachloroethylene to trichloroethylene, dichloroethylene, vinyl chloride, and carbon dioxide under methanogenic conditions.
    Appl Environ Microbiol. 1985 May;49(5):1080-3 PMID: 3923927
  23. Toluene degradation by Pseudomonas putida F1: genetic organization of the tod operon.
    Appl Environ Microbiol. 1988 Jun;54(6):1498-503 PMID: 2843094
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.55.12.3162-3166.1989
Published
1989-12-00
Pages
3162-6
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC203240
Subset
IM
Grants
NIGMS NIH HHS · 1 F32 GM13091 · United States
NIGMS NIH HHS · GM29909 · United States
NIGMS NIH HHS · GM41235 · United States
External Links
PubMed source DOI: 10.1128/aem.55.12.3162-3166.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2