Molecular characterization of the gene encoding SHV-3 beta-lactamase responsible for transferable cefotaxime resistance in clinical isolates of Klebsiella pneumoniae.

Antimicrobial agents and chemotherapy ·Vol. 33 ·No. 12 ·1989-12-00 ·Pages 2096-100

Nicolas MH, Jarlier V, Honore N, Philippon A, Cole ST

Abstract

In Klebsiella pneumoniae 86-4, cefotaxime resistance was due to a transferable broad-spectrum beta-lactamase, SHV-3. The plasmid-borne gene encoding SHV-3 has been cloned, and the primary structure of the enzyme was deduced from its nucleotide sequence. SHV-3 differs from SHV-1 in two positions. The extended substrate profile of SHV-3 probably results from the substitution of Ser-213 for Gly, as in SHV-2, whereas replacement of Arg-180 by Leu resulted in a decrease in the pI from 7.6 to 7.0. The blashv-3 gene is highly homologous (92% DNA sequence identity) with the chromosomal gene coding for LEN-1 beta-lactamase of K. pneumoniae, suggesting that the origin of the SHV-encoding genes now present on many plasmids may be chromosomal.

MeSH Terms

Base Sequence Cefotaxime/pharmacology Cloning, Molecular Cross Infection/microbiology Culture Media Drug Resistance, Microbial/genetics Electrophoresis, Agar Gel Escherichia coli/drug effects,genetics Klebsiella Infections/microbiology Klebsiella pneumoniae/drug effects,genetics Molecular Sequence Data Plasmids Restriction Mapping beta-Lactamases/genetics

Chemicals

Culture Media beta-lactamase SHV-3 beta-Lactamases Cefotaxime

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Nicolas M H

Laboratoire de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.

Jarlier V

Honore N

Philippon A

Cole S T

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.33.12.2096

Published

1989-12-00

Pages

2096-100

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC172828

Subset

External Links

PubMed source DOI: 10.1128/AAC.33.12.2096 PMC Full Text View journal details

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Molecular characterization of the gene encoding SHV-3 beta-lactamase responsible for transferable cefotaxime resistance in clinical isolates of Klebsiella pneumoniae.

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