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PMID: 26916 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Hemopoietic stem cell transplantation using mouse bone marrow and spleen cells fractionated by lectins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 6 ·1978-06-00 ·Pages 2933-6

Reisner Y, Itzicovitch L, Meshorer A, Sharon N

Abstract

Mouse bone marrow and spleen cells were fractionated with the aid of soybean agglutinin and peanut agglutinin. A test for spleen colony-forming units in the isolated fractions showed that the hemopoietic stem cells are agglutinated by both of these lectins. The capacity of the agglutinated fractions to reconstitute lethally irradiated allogeneic mice was investigated. A sequential fractionation of splenocytes from SWR donors by soybean agglutinin and peanut agglutinin, or a single fractionation by soybean agglutinin of splenocytes from BALB/c donors, afforded a cell fraction that successfully reconstituted lethally irradiated (BALB/c X C57BL/6)F1 mice, without complications due to graft-versus-host reaction.

MeSH Terms

Animals Bone Marrow Cells Bone Marrow Transplantation Cell Aggregation Cell Separation/methods Female Graft vs Host Reaction Hematopoietic Stem Cell Transplantation Lectins Mice Radiation Chimera Spleen/cytology,transplantation Transplantation, Homologous

Chemicals

Lectins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Reisner Y

Itzicovitch L

Meshorer A

Sharon N

References (10)

10 references, click to expand

Interaction of peanut agglutinin, a lectin specific for nonreducing terminal D-galactosyl residues, with embryonal carcinoma cells.
Dev Biol. 1977 Nov;61(1):20-7 PMID: 924023
A direct measurement of the radiation sensitivity of normal mouse bone marrow cells.
Radiat Res. 1961 Feb;14:213-22 PMID: 13776896
Subpopulations of T-lymphocytes. Physical separation, functional specialisation and differentiation pathways of sub-sets of thymocytes and thymus-dependent peripheral lymphocytes.
Transplant Rev. 1975;25:163-210 PMID: 1103380
Graft versus host reactions. Their natural history, and applicability as tools of research.
Prog Allergy. 1962;6:349-467 PMID: 14037719
Separation of mouse thymocytes into two subpopulations by the use of peanut agglutinin.
Cell Immunol. 1976 Jul;25(1):129-34 PMID: 9202
The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea).
J Biol Chem. 1975 Nov 10;250(21):8518-23 PMID: 811657
Use of soybean agglutinin for the separation of mouse B and T lymphocytes.
Biochem Biophys Res Commun. 1976 Oct 18;72(4):1585-91 PMID: 11794
Purification of soybean agglutinin by affinity chromatography On sepharose-N-epsilon-aminocaproyl-beta-D-galactopyranosylamine.
FEBS Lett. 1972 Aug 1;24(2):193-196 PMID: 11946670
Allogeneic bone marrow transplantation after elimination of immunocompetent cells by means of density gradient centrifugation.
Transplant Proc. 1971 Mar;3(1):666-8 PMID: 4398506
The double barrier in bone marrow transplantation.
Semin Hematol. 1974 Jul;11(3):325-40 PMID: 4151846

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.6.2933

Published

1978-06-00

Pages

2933-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392680

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.6.2933 PMC Full Text View journal details

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Hemopoietic stem cell transplantation using mouse bone marrow and spleen cells fractionated by lectins.

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