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题目:: TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues.
作者:: Matsuura(Katsuhisa),Seta(Hiroyoshi),Haraguchi(Yuji),Alsayegh(Khaled),Sekine(Hidekazu),Shimizu(Tatsuya),Hagiwara(Nobuhisa),Yamazaki(Kenji),Okano(Teruo)
状态:: 发布时间2016-02-18 , 更新时间 2016-02-27
期刊:: Sci Rep
摘要:: The development of a suitable strategy for eliminating remaining undifferentiated cells is indispensable for the use of human-induced pluripotent stem (iPS) cell-derived cells in regenerative medicine. Here, we show for the first time that TRPV-1 activation through transient culture at 42 °C in combination with agonists is a simple and useful strategy to eliminate iPS cells from bioengineered cardiac cell sheet tissues. When human iPS cells were cultured at 42 °C, almost all cells disappeared by 48 hours through apoptosis. However, iPS cell-derived cardiomyocytes and fibroblasts maintained transcriptional and protein expression levels, and cardiac cell sheets were fabricated after reducing the temperature. TRPV-1 expression in iPS cells was upregulated at 42 °C, and iPS cell death at 42 °C was TRPV-1-dependent. Furthermore, TRPV-1 activation through thermal or agonist treatment eliminated iPS cells in cardiac tissues for a final concentration of 0.4% iPS cell contamination. These findings suggest that the difference in tolerance to TRPV-1 activation between iPS cells and iPS cell-derived cardiac cells could be exploited to eliminate remaining iPS cells in bioengineered cell sheet tissues, which will further reduce the risk of tumour formation.
语言:: eng
DOI:

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