Inactivation of the Escherichia coli heat-labile enterotoxin by in vitro mutagenesis of the A-subunit gene.

European journal of biochemistry ·Vol. 183 ·No. 2 ·1989-08-01 ·Pages 311-6

Harford S, Dykes CW, Hobden AN, Read MJ, Halliday IJ

Abstract

In vitro mutagenesis of the LTA gene, encoding the A subunit of the Escherichia coli heat-labile enterotoxin, has been used to obtain A subunits deficient in enzymic activity. One inactive A-subunit mutant which contained two amino acid substitutions, was shown to associate with native B subunits to form a holotoxoid lacking toxin activity. A serine to phenylalanine mutation appears to be responsible for the loss of toxicity.

MeSH Terms

Amino Acid Sequence Animals Bacterial Toxins/genetics,isolation & purification,pharmacology Base Sequence Biological Assay Chromatography, Affinity Cloning, Molecular Deoxyribonuclease EcoRI Deoxyribonucleases, Type II Site-Specific Enterotoxins/genetics,isolation & purification,pharmacology Escherichia coli/genetics Escherichia coli Proteins Ileum/metabolism Immunosorbent Techniques Molecular Sequence Data Mutation Plasmids Rabbits

Chemicals

Bacterial Toxins Enterotoxins Escherichia coli Proteins heat-labile enterotoxin, E coli Deoxyribonuclease EcoRI endodeoxyribonuclease XBAI Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Harford S

Fermentation Development Department, Glaxochem, Ulverston.

Dykes C W

Hobden A N

Read M J

Halliday I J

Article Info

Journal

European journal of biochemistry

Abbr.

Eur J Biochem

ISSN

0014-2956

DOI

10.1111/j.1432-1033.1989.tb14930.x

Published

1989-08-01

Pages

311-6

Language

English

Region

England

NLM ID

0107600

Subset

External Links

PubMed source DOI: 10.1111/j.1432-1033.1989.tb14930.x

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Inactivation of the Escherichia coli heat-labile enterotoxin by in vitro mutagenesis of the A-subunit gene.

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