| GeneLibs

题目:: Cell-Cycle-Dependent Reconfiguration of the DNA Methylome during Terminal Differentiation of Human B Cells into Plasma Cells.
作者:: Caron(Gersende),Hussein(Mourad),Kulis(Marta),Delaloy(Céline),Chatonnet(Fabrice),Pignarre(Amandine),Avner(Stéphane),Lemarié(Maud),Mahé(Elise A),Verdaguer-Dot(Núria),Queirós(Ana C),Tarte(Karin),Martín-Subero(José I),Salbert(Gilles),Fest(Thierry)
状态:: 发布时间2015-11-14 , 更新时间 2015-11-14
期刊:: Cell Rep
摘要:: Molecular mechanisms underlying terminal differentiation of B cells into plasma cells are major determinants of adaptive immunity but remain only partially understood. Here we present the transcriptional and epigenomic landscapes of cell subsets arising from activation of human naive B cells and differentiation into plasmablasts. Cell proliferation of activated B cells was linked to a slight decrease in DNA methylation levels, but followed by a committal step in which an S phase-synchronized differentiation switch was associated with an extensive DNA demethylation and local acquisition of 5-hydroxymethylcytosine at enhancers and genes related to plasma cell identity. Downregulation of both TGF-?1/SMAD3 signaling and p53 pathway supported this final step, allowing the emergence of a CD23-negative subpopulation in transition from B cells to plasma cells. Remarkably, hydroxymethylation of PRDM1, a gene essential for plasma cell fate, was coupled to progression in S phase, revealing an intricate connection among cell cycle, DNA (hydroxy)methylation, and cell fate determination.
语言:: eng
DOI:: 10.1016/j.celrep.2015.09.051

文献库文献相关信息

DataTable pData

联系方式

微信公众号

文献库 文献相关信息

DataTable pData

联系方式

微信公众号

文献库文献相关信息