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PMID: 2653743 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Release of neutrophil chemotactic factors from gastric tissue. Initial biochemical characterization.

Digestive diseases and sciences ·Vol. 34 ·No. 5 ·1989-05-00 ·Pages 681-7

Kozol RA, Downes RJ, Kreutzer DL, Wentzel S, Rossomando E, Elgebaly SA

Abstract

This study was designed to characterize neutrophil chemotactic factors released by gastric tissue. Full-thickness rabbit stomach (organ culture) was prepared and incubated in Ringer's solution at 37 degrees C. Culture supernatants were collected at 1, 2, 3, and 4 hr and assayed for neutrophil chemotactic activity in modified Boyden chambers. High levels of chemotactic activity were seen at 3 hr of incubation. Antral and fundic tissue were equally capable of producing neutrophil chemotactic activity. In addition, high levels of activity were seen from both the serosal and mucosal surfaces. Initial biochemical characterization of these gastric-derived factors revealed that: (1) a majority of the activity (80-90%) exhibited molecular weight values of greater than 300 kDa, (2) the chemotactic activity was heat stable but was partially reduced by treatment with a protease, subtilisin (37% inhibition), and (3) 70-80% of the activity in the supernatants was extracted into organic solvent (ethyl acetate). These factors may prove to be important in recruitment of neutrophils to areas of gastric injury.

MeSH Terms
Animals Chemotactic Factors/analysis,isolation & purification,metabolism,pharmacology Chemotaxis, Leukocyte/drug effects Chromatography, High Pressure Liquid Electrophoresis, Gel, Two-Dimensional Gastric Mucosa/metabolism Interleukin-8 Isoelectric Focusing Molecular Weight Neutrophils/drug effects,physiology Organ Culture Techniques Rabbits Solubility Time Factors
Chemicals
Chemotactic Factors Interleukin-8
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kozol R A
Department of Surgery, University of Connecticut School of Medicine, Farmington 06032.
Downes R J
Kreutzer D L
Wentzel S
Rossomando E
Elgebaly S A
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Article Info
Journal
Digestive diseases and sciences
Abbr.
Dig Dis Sci
ISSN
0163-2116
DOI
10.1007/BF01540338
Published
1989-05-00
Pages
681-7
Language
English
Region
United States
NLM ID
7902782
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01540338 View journal details
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