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题目:: Neural cells play an inhibitory role in pancreatic differentiation of pluripotent stem cells.
作者:: Nakashima(Ryutaro),Morooka(Mayu),Shiraki(Nobuaki),Sakano(Daisuke),Ogaki(Soichiro),Kume(Kazuhiko),Kume(Shoen)
状态:: 发布时间2016-01-15 , 更新时间 2016-11-10
期刊:: Genes Cells
摘要:: Pancreatic endocrine β-cells derived from embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have received attention as screening systems for therapeutic drugs and as the basis for cell-based therapies. Here, we used a 12-day β-cell differentiation protocol for mouse ES cells and obtained several hit compounds that promoted β-cell differentiation. One of these compounds, mycophenolic acid (MPA), effectively promoted ES cell differentiation with a concomitant reduction of neuronal cells. The existence of neural cell-derived inhibitory humoral factors for β-cell differentiation was suggested using a co-culture system. Based on gene array analysis, we focused on the Wnt/β-catenin pathway and showed that the Wnt pathway inhibitor reversed MPA-induced β-cell differentiation. Wnt pathway activation promoted β-cell differentiation also in human iPS cells. Our results showed that Wnt signaling activation positively regulates β-cell differentiation, and represent a downstream target of the neural inhibitory factor.
语言:: eng
DOI:

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