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PMID: 26509874 Published · epublish English Journal Article

A Higher Frequency of CD14+ CD169+ Monocytes/Macrophages in Patients with Colorectal Cancer.

PloS one ·Vol. 10 ·No. 10 ·2015-00-00 ·Pages e0141817

Li C, Luo X, Lin Y, Tang X, Ling L, Wang L, Jiang Y

Abstract

Monocytes and macrophages can infiltrate into tumor microenvironment and regulate the progression of tumors. This study aimed at determining the frequency of different subsets of circulating monocytes and tumor infiltrating macrophages (TIMs) in patients with colorectal cancer (CRC). The frequency of different subsets of circulating monocytes was characterized in 46 CRC patients and 22 healthy controls (HC) by flow cytometry. The frequency of different subsets of macrophages was analyzed in TIMs from 30 tumor tissues and in lamina propria mononuclear cells (LPMCs) from 12 non-tumor tissues. The concentrations of plasma cytokines and carcinoembryonic antigen (CEA) were determined. The potential association of these measures with the values of clinical parameters was analyzed. In comparison with that in the HC, the percentages of circulating CD14+ CD169+, CD14+ CD169+ CD163+ and CD14+ CD169+ CD206+ monocytes and TIMs CD14+ CD169+ as well as IL-10+ CD14+ CD169+, but not IL-12+ CD14+ CD169+ macrophages were significantly increased, accompanied by higher levels of plasma IL-10 in the CRC patients. The percentages of CD14+ CD169+ circulating monocytes and TIM macrophages were associated with the stage of disease and correlated positively with the levels of plasma IL-10 and CEA in CRC patients. Our data suggest that an increase in the frequency of CD14+ CD169+ cells may be associated with the development and progression of CRC and is concomitant rise of both, pro-tumor (M2-like, IL-10 producing) and anti-tumor (M1-like, IL-12 producing) monocytes and infiltrating macrophages. The frequency of CD14+ CD169+ circulating monocytes and infiltrating macrophages may serve as a biomarker for evaluating the pathogenic degrees of CRC.

MeSH Terms
Adult Aged Antigens, CD/metabolism Case-Control Studies Colorectal Neoplasms/immunology,metabolism,pathology Female Humans Immunophenotyping Intestinal Mucosa/immunology,metabolism,pathology Leukocyte Count Lipopolysaccharide Receptors/metabolism Macrophages/immunology,metabolism Male Middle Aged Monocytes/immunology,metabolism Neoplasm Grading Neoplasm Staging Phenotype Sialic Acid Binding Ig-like Lectin 1/metabolism
Chemicals
Antigens, CD Lipopolysaccharide Receptors Sialic Acid Binding Ig-like Lectin 1
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Li Chenguang
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Luo Xiaofan
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Lin Yuyang
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Tang Xiuqi
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Ling Limian
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Wang Lei
Department of Colorectal & Anal Surgery, The First Hospital of Jilin University, Changchun, China.
Jiang Yanfang
Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, China; Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital of Jilin University, Changchun, China.
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0141817
Published
2015-00-00
Epub
2015-00-28
Pages
e0141817
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC4625021
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0141817 PMC Full Text View journal details
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