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PMID: 26496607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

EIN2-directed translational regulation of ethylene signaling in Arabidopsis.

Cell ·Vol. 163 ·No. 3 ·2015-10-22 ·Pages 670-83

Li W, Ma M, Feng Y, Li H, Wang Y, Ma Y, Li M, An F, Guo H

Abstract

Ethylene is a gaseous phytohormone that plays vital roles in plant growth and development. Previous studies uncovered EIN2 as an essential signal transducer linking ethylene perception on ER to transcriptional regulation in the nucleus through a "cleave and shuttle" model. In this study, we report another mechanism of EIN2-mediated ethylene signaling, whereby EIN2 imposes the translational repression of EBF1 and EBF2 mRNA. We find that the EBF1/2 3' UTRs mediate EIN2-directed translational repression and identify multiple poly-uridylates (PolyU) motifs as functional cis elements of 3' UTRs. Furthermore, we demonstrate that ethylene induces EIN2 to associate with 3' UTRs and target EBF1/2 mRNA to cytoplasmic processing-body (P-body) through interacting with multiple P-body factors, including EIN5 and PABs. Our study illustrates translational regulation as a key step in ethylene signaling and presents mRNA 3' UTR functioning as a "signal transducer" to sense and relay cellular signaling in plants. VIDEO ABSTRACT.

MeSH Terms

Arabidopsis/metabolism Arabidopsis Proteins/genetics,metabolism Ethylenes/metabolism Exoribonucleases/metabolism F-Box Proteins/genetics Gene Expression Regulation, Plant Nucleic Acid Conformation Plant Proteins/metabolism Protein Biosynthesis RNA, Messenger/chemistry,metabolism Receptors, Cell Surface/metabolism

Chemicals

Arabidopsis Proteins EBF1 protein, Arabidopsis EIN2 protein, Arabidopsis Ethylenes F-Box Proteins Plant Proteins RNA, Messenger Receptors, Cell Surface Xrn1 protein, plant ethylene Exoribonucleases

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Li Wenyang