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PMID: 2649386 Published · ppublish English Journal Article

Isolation of verocytotoxin-producing Escherichia coli from milk filters in south-western Ontario.

Epidemiology and infection ·Vol. 102 ·No. 2 ·1989-04-00 ·Pages 253-60

Clarke RC, McEwen SA, Gannon VP, Lior H, Gyles CL

Abstract

A total of 1012 milk filters collected from 498 dairy farms in south-western Ontario during three study periods (December 1985-March 1986) were tested for the presence of verocytotoxin-producing Escherichia coli (VTEC). VTEC were detected and isolated using a Vero cell assay. Supernatants from 20 of the milk filter cultures had verocytotoxic activity and 7 VTEC strains were isolated from these positive samples. The prevalence of VTEC in the samples in each of the three study periods were 0.44, 0.65 and 0.99% respectively. All seven VTEC strains isolated were sensitive to commonly used antimicrobial agents. The serotypes of these strains were O 26.H11, O43.H2, O 153.H25, O ?. H8, O? .H19, O ?. non-motile, and Orough.H19. Two of these serotypes (O 153.H25 and O 26.H11) have previously been associated with disease in humans.

MeSH Terms
Animals Bacterial Toxins/biosynthesis Bacterial Typing Techniques Dairying/instrumentation Escherichia coli/classification,isolation & purification,metabolism Filtration/instrumentation Milk/microbiology Ontario Serotyping Shiga Toxin 1
Chemicals
Bacterial Toxins Shiga Toxin 1
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Clarke R C
Health of Animals Laboratory, Agriculture Canada, Guelph, Ontario.
McEwen S A
Gannon V P
Lior H
Gyles C L
References (29)
29 references, click to expand
  1. Sporadic cases of hemorrhagic colitis associated with Escherichia coli O157:H7. Clinical, epidemiologic, and bacteriologic features.
    Ann Intern Med. 1984 Dec;101(6):738-42 PMID: 6388450
  2. Vero cell toxins in Escherichia coli and related bacteria: transfer by phage and conjugation and toxic action in laboratory animals, chickens and pigs.
    J Gen Microbiol. 1983 Oct;129(10):3121-37 PMID: 6418852
  3. Shiga-like toxin production from Escherichia coli associated with calf diarrhoea.
    Vet Rec. 1985 Feb 23;116(8):217-8 PMID: 3885557
  4. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli.
    J Infect Dis. 1985 May;151(5):775-82 PMID: 3886804
  5. Results of a screening method used in a 12-month stool survey for Escherichia coli O157:H7.
    J Infect Dis. 1985 Oct;152(4):775-7 PMID: 3900234
  6. Hemorrhagic colitis with Escherichia coli O157:H7 preceding adult hemolytic uremic syndrome.
    Arch Intern Med. 1985 Dec;145(12):2215-7 PMID: 3907563
  7. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis.
    J Clin Microbiol. 1986 May;23(5):869-72 PMID: 3519658
  8. Natural and experimental infection with an attaching and effacing strain of Escherichia coli in calves.
    Infect Immun. 1986 Aug;53(2):339-46 PMID: 3525410
  9. Hemolytic uremic syndrome and diarrhea associated with Escherichia coli O157:H7 in a day care center.
    J Pediatr. 1986 Aug;109(2):287-91 PMID: 3525791
  10. Isolation of Escherichia coli O157:H7 from dairy cattle associated with two cases of haemolytic uraemic syndrome.
    Lancet. 1986 Nov 1;2(8514):1043 PMID: 2877210
  11. Bovine reservoir for verotoxin-producing Escherichia coli O157:H7.
    Lancet. 1987 Jan 10;1(8524):98 PMID: 2879192
  12. Shiga-like toxin production and attaching effacing activity of Escherichia coli associated with calf diarrhea.
    Am J Vet Res. 1987 May;48(5):743-8 PMID: 3296889
  13. Laboratory investigation of outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7.
    J Clin Microbiol. 1987 Jun;25(6):1043-7 PMID: 3298310
  14. A DNA probe to identify enterohemorrhagic Escherichia coli of O157:H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome.
    J Infect Dis. 1987 Jul;156(1):175-82 PMID: 3298451
  15. Cattle as reservoir of verotoxin-producing Escherichia coli O157:H7.
    Lancet. 1987 Aug 1;2(8553):276 PMID: 2886741
  16. Frequency of Escherichia coli O157:H7 isolation from stool specimens.
    Can J Microbiol. 1987 Oct;33(10):914-5 PMID: 3319115
  17. Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry.
    Appl Environ Microbiol. 1987 Oct;53(10):2394-6 PMID: 3322190
  18. Studies in gnotobiotic piglets on non-O157:H7 Escherichia coli serotypes isolated from patients with hemorrhagic colitis.
    Gastroenterology. 1988 Mar;94(3):590-7 PMID: 3276573
  19. The prevalence, incidence, geographical distribution, antimicrobial sensitivity patterns and plasmid profiles of milk filter Salmonella isolates from Ontario dairy farms.
    Can J Vet Res. 1988 Jan;52(1):18-22 PMID: 3349397
  20. Vero cytotoxin-producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes.
    J Med Microbiol. 1988 Apr;25(4):237-43 PMID: 3282072
  21. Epidemiology of sporadic diarrhea due to verocytotoxin-producing Escherichia coli: a two-year prospective study.
    J Infect Dis. 1988 May;157(5):1054-7 PMID: 3283256
  22. Characteristics of verotoxigenic Escherichia coli from pigs.
    Can J Vet Res. 1988 Jul;52(3):331-7 PMID: 3048621
  23. The transmissible nature of enterotoxin production in a human enteropathogenic strain of Escherichia coli.
    J Med Microbiol. 1971 Aug;4(3):301-5 PMID: 4940299
  24. Vero response to a cytotoxin of Escherichia coli.
    Infect Immun. 1977 Dec;18(3):775-9 PMID: 338490
  25. Cytotoxic Escherichia coli O157:H7 associated with haemorrhagic colitis in Canada.
    Lancet. 1983 Jan 1;1(8314-5):76 PMID: 6129412
  26. Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools.
    Lancet. 1983 Mar 19;1(8325):619-20 PMID: 6131302
  27. Hemorrhagic colitis associated with a rare Escherichia coli serotype.
    N Engl J Med. 1983 Mar 24;308(12):681-5 PMID: 6338386
  28. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype.
    J Clin Microbiol. 1983 Sep;18(3):512-20 PMID: 6355145
  29. Survival and growth characteristics of Escherichia coli associated with hemorrhagic colitis.
    Appl Environ Microbiol. 1984 Oct;48(4):855-6 PMID: 6391379
Article Info
Journal
Epidemiology and infection
Abbr.
Epidemiol Infect
ISSN
0950-2688
DOI
10.1017/s0950268800029927
Published
1989-04-00
Pages
253-60
Language
English
Region
England
NLM ID
8703737
PMCID
PMC2249426
Subset
IM
External Links
PubMed source DOI: 10.1017/s0950268800029927 PMC Full Text View journal details
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