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PMID: 2649078 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tissue distribution of a novel neurotensin-degrading metallopeptidase. An immunological approach using monospecific polyclonal antibodies.

The Biochemical journal ·Vol. 257 ·No. 2 ·1989-01-15 ·Pages 549-54

Checler F, Barelli H, Vincent JP

Abstract

A monospecific polyclonal antiserum was raised against a recently purified rat brain neurotensin-degrading metallopeptidase. The purified IgG fraction immunoprecipitated the peptidase and inhibited its proteolytic activity. Western blot analyses revealed that the immune fraction recognizes only one protein in rat brain homogenates, and this corresponds closely to the purified enzyme. The IgG displayed a restricted specificity towards the peptidase from murine origin. In the rat, the neurotensin-degrading enzyme was widely distributed throughout peripheral organs with the noticeable exception of the duodenum. In addition, the peptidase was detected in various cell lines or membrane preparations of neural or extraneural origin in which it had been previously characterized by means of biochemical methods. In light of this widespread distribution, the putative role of the peptidase in the metabolism of neuropeptides is discussed.

MeSH Terms
Animals Antibodies Brain/enzymology,immunology Brain Chemistry Immunoenzyme Techniques Immunoglobulin G/metabolism Metalloendopeptidases/metabolism Neuropeptides/metabolism Neurotensin/metabolism Rats
Chemicals
Antibodies Immunoglobulin G Neuropeptides Neurotensin Metalloendopeptidases neurotensin-degrading enzyme
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Checler F
Centre de Biochimie, CNRS, Université de Nice, Faculté des Sciences, France.
Barelli H
Vincent J P
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18 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2570549
Published
1989-01-15
Pages
549-54
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135613
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2570549 PMC Full Text View journal details
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