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PMID: 264685 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Microcell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 1 ·1977-01-00 ·Pages 319-23

Fournier RE, Ruddle FH

Abstract

In this report, we describe the production and characterization of proliferating hybrid cell populations generated by fusion of murine microcells with intact mouse, Chinese hamster, and human recipient cells. The microcell hybrids so produced contained one to five intact murine chromosomes derived from the microcell donor. these transferred chromosomes were maintained as functioning genetic elements in the hybrid cells. Our results firmly establish subnuclear particle-mediated chromosome transfer as a valid somatic cell genetic tool.

MeSH Terms
Animals Cell Fusion/methods Cells, Cultured Chromosomes/transplantation Cricetinae Demecolcine/pharmacology Genetic Engineering Humans Hybrid Cells/enzymology,ultrastructure Karyotyping L Cells/drug effects,ultrastructure Mice Transplantation, Heterologous Transplantation, Homologous
Chemicals
Demecolcine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fournier R E
Ruddle F H
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.1.319
Published
1977-01-00
Pages
319-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393251
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.1.319 PMC Full Text View journal details
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